Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 31461 | 3' | -38.9 | NC_006649.1 | + | 31344 | 0.85 | 0.641487 |
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Target: 5'- uUCC-AUAAUAUUAUGGaAAUGGUUCCc -3' miRNA: 3'- -AGGaUAUUAUAAUAUCcUUACCAAGG- -5' |
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| 31461 | 3' | -38.9 | NC_006649.1 | + | 31527 | 0.81 | 0.844701 |
|
Target: 5'- cUCCUAUAAUugUAUAGGAAcuGUUCCc -3' miRNA: 3'- -AGGAUAUUAuaAUAUCCUUacCAAGG- -5' |
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| 31461 | 3' | -38.9 | NC_006649.1 | + | 31480 | 0.79 | 0.915061 |
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Target: 5'- uUCCcAUAAUAgUAUAGGAAUaGUUCCc -3' miRNA: 3'- -AGGaUAUUAUaAUAUCCUUAcCAAGG- -5' |
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| 31461 | 3' | -38.9 | NC_006649.1 | + | 31571 | 0.77 | 0.952334 |
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Target: 5'- uUCCUAUA--AUUAUGGGAAcaGUUCCc -3' miRNA: 3'- -AGGAUAUuaUAAUAUCCUUacCAAGG- -5' |
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| 31461 | 3' | -38.9 | NC_006649.1 | + | 5564 | 0.77 | 0.957243 |
|
Target: 5'- uUCCcAUAAaAUUAUGGGAAUaGUUCCc -3' miRNA: 3'- -AGGaUAUUaUAAUAUCCUUAcCAAGG- -5' |
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| 31461 | 3' | -38.9 | NC_006649.1 | + | 31614 | 0.71 | 0.998619 |
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Target: 5'- uUCCUAUAAUugccUGGGAAcuGUUCCc -3' miRNA: 3'- -AGGAUAUUAuaauAUCCUUacCAAGG- -5' |
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| 31461 | 3' | -38.9 | NC_006649.1 | + | 5519 | 1.16 | 0.014098 |
|
Target: 5'- uUCCUAUAAUAUUAUAGGAAUGGUUCCc -3' miRNA: 3'- -AGGAUAUUAUAAUAUCCUUACCAAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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