miRNA: 3'- -AUCaauCUGACCGGUCAA---UGAUCGGU- -5'
miRNA: 3'- aUCAAUCUGa--CCG-GUCAaUGAuCGGU- -5'
miRNA: 3'- -AUCAAucUGAcCGGUCAAUGAUCGGU- -5'
miRNA: 3'- -AUCaAUC--UGACCGGUCAA--UGAUC-GGu -5'
miRNA: 3'- -AUCAAUcUGACCGGUCAAUGAUCGGU- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31463 | 5' | -50.6 | NC_006649.1 | + | 25569 | 0.66 | 0.745793 |
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Target: 5'- aUAGgu-GACUGGUUAGUUagaGCUGGUUAu -3' miRNA: 3'- -AUCaauCUGACCGGUCAA---UGAUCGGU- -5' |
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| 31463 | 5' | -50.6 | NC_006649.1 | + | 22408 | 0.67 | 0.710854 |
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Target: 5'- aGGUUAGACacaGGCgCGGUcACU-GCCAu -3' miRNA: 3'- aUCAAUCUGa--CCG-GUCAaUGAuCGGU- -5' |
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| 31463 | 5' | -50.6 | NC_006649.1 | + | 18101 | 0.68 | 0.663025 |
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Target: 5'- cUAGUU--ACUuGCCAGUUACUGGUUAg -3' miRNA: 3'- -AUCAAucUGAcCGGUCAAUGAUCGGU- -5' |
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| 31463 | 5' | -50.6 | NC_006649.1 | + | 18134 | 0.7 | 0.542813 |
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Target: 5'- uUGGcUAGuaACUGGCCAGUUcaACUAGaCCu -3' miRNA: 3'- -AUCaAUC--UGACCGGUCAA--UGAUC-GGu -5' |
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| 31463 | 5' | -50.6 | NC_006649.1 | + | 18075 | 0.95 | 0.011898 |
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Target: 5'- cUAGUUGaACUGGCCAGUUACUAGCCAa -3' miRNA: 3'- -AUCAAUcUGACCGGUCAAUGAUCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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