Results 21 - 28 of 28 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31465 | 5' | -44.4 | NC_006649.1 | + | 31540 | 0.79 | 0.476337 |
Target: 5'- gUGGGAACUAUUCCUAUAcUAuUAUGGg -3' miRNA: 3'- -ACCCUUGGUAAGGGUAUuAUcAUAUC- -5' |
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31465 | 5' | -44.4 | NC_006649.1 | + | 5531 | 0.78 | 0.547405 |
Target: 5'- -aGGuACCAUUCCCAUAAUAuUAUGGg -3' miRNA: 3'- acCCuUGGUAAGGGUAUUAUcAUAUC- -5' |
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31465 | 5' | -44.4 | NC_006649.1 | + | 31355 | 0.73 | 0.811888 |
Target: 5'- -aGGAAUaGUUUCUAUAAUAGUAUAGg -3' miRNA: 3'- acCCUUGgUAAGGGUAUUAUCAUAUC- -5' |
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31465 | 5' | -44.4 | NC_006649.1 | + | 5485 | 0.73 | 0.811888 |
Target: 5'- -aGGAAUaGUUUCUAUAAUAGUAUAGg -3' miRNA: 3'- acCCUUGgUAAGGGUAUUAUCAUAUC- -5' |
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31465 | 5' | -44.4 | NC_006649.1 | + | 31697 | 0.72 | 0.852398 |
Target: 5'- aUGGaAACUGUUCCCAUAAUuuUAUGGg -3' miRNA: 3'- -ACCcUUGGUAAGGGUAUUAucAUAUC- -5' |
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31465 | 5' | -44.4 | NC_006649.1 | + | 31649 | 0.68 | 0.97043 |
Target: 5'- -aGGAACUAUUCCCAUAAa------- -3' miRNA: 3'- acCCUUGGUAAGGGUAUUaucauauc -5' |
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31465 | 5' | -44.4 | NC_006649.1 | + | 31587 | 0.68 | 0.973809 |
Target: 5'- aUGGGAACaGUUCCUAUAcaauUAUAGg -3' miRNA: 3'- -ACCCUUGgUAAGGGUAUuaucAUAUC- -5' |
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31465 | 5' | -44.4 | NC_006649.1 | + | 5441 | 1.12 | 0.004905 |
Target: 5'- aUGGGAACCAUUCCCAUAAUGGUAUAGg -3' miRNA: 3'- -ACCCUUGGUAAGGGUAUUAUCAUAUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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