Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31465 | 5' | -44.4 | NC_006649.1 | + | 5624 | 0.9 | 0.127252 |
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Target: 5'- aUGGGAACUAUUCCCAUAAUuuUAUGGg -3' miRNA: 3'- -ACCCUUGGUAAGGGUAUUAucAUAUC- -5' |
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| 31465 | 5' | -44.4 | NC_006649.1 | + | 31403 | 0.91 | 0.101804 |
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Target: 5'- aUGGGAACCAUUUCCAUAAUAuUAUGGa -3' miRNA: 3'- -ACCCUUGGUAAGGGUAUUAUcAUAUC- -5' |
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| 31465 | 5' | -44.4 | NC_006649.1 | + | 31495 | 0.92 | 0.092434 |
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Target: 5'- aUGGaAACCAUUCCUAUAAUAGUAUAGg -3' miRNA: 3'- -ACCcUUGGUAAGGGUAUUAUCAUAUC- -5' |
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| 31465 | 5' | -44.4 | NC_006649.1 | + | 5579 | 0.94 | 0.07129 |
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Target: 5'- aUGGGAACCAUUCCUAUAAUAuUAUAGg -3' miRNA: 3'- -ACCCUUGGUAAGGGUAUUAUcAUAUC- -5' |
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| 31465 | 5' | -44.4 | NC_006649.1 | + | 31311 | 0.96 | 0.048055 |
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Target: 5'- aUGGaAACCAUUCCCAUAAUGGUAUAGg -3' miRNA: 3'- -ACCcUUGGUAAGGGUAUUAUCAUAUC- -5' |
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| 31465 | 5' | -44.4 | NC_006649.1 | + | 31449 | 0.99 | 0.033355 |
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Target: 5'- aUGGGAACCAUUCCCAUAAUAuUAUAGg -3' miRNA: 3'- -ACCCUUGGUAAGGGUAUUAUcAUAUC- -5' |
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| 31465 | 5' | -44.4 | NC_006649.1 | + | 5441 | 1.12 | 0.004905 |
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Target: 5'- aUGGGAACCAUUCCCAUAAUGGUAUAGg -3' miRNA: 3'- -ACCCUUGGUAAGGGUAUUAUCAUAUC- -5' |
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| 31465 | 5' | -44.4 | NC_006649.1 | + | 31470 | 1.12 | 0.004432 |
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Target: 5'- aUGGGAACCAUUCCCAUAAUAGUAUAGg -3' miRNA: 3'- -ACCCUUGGUAAGGGUAUUAUCAUAUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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