Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31466 | 3' | -41.9 | NC_006649.1 | + | 5524 | 0.94 | 0.113829 |
Target: 5'- aUUCCCAUAAUaUUAUGGGAuUAGUUCCc -3' miRNA: 3'- -AAGGGUAUUA-AAUACCCUuAUCAAGG- -5' |
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31466 | 3' | -41.9 | NC_006649.1 | + | 31388 | 0.96 | 0.084638 |
Target: 5'- aUUCCUAUAAUaUUAUGGGAAUGGUUCCc -3' miRNA: 3'- -AAGGGUAUUA-AAUACCCUUAUCAAGG- -5' |
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31466 | 3' | -41.9 | NC_006649.1 | + | 5563 | 0.97 | 0.074089 |
Target: 5'- gUUCCCAUAAaaUUAUGGGAAUAGUUCCc -3' miRNA: 3'- -AAGGGUAUUa-AAUACCCUUAUCAAGG- -5' |
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31466 | 3' | -41.9 | NC_006649.1 | + | 31591 | 1.12 | 0.008615 |
Target: 5'- gUUCCCAUAAUUUAUGGGAAUAGUUCCu -3' miRNA: 3'- -AAGGGUAUUAAAUACCCUUAUCAAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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