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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31470 | 3' | -57.7 | NC_006650.1 | + | 14707 | 0.66 | 0.314489 |
Target: 5'- --cACGCUA--UGGCCGUcgcacaUGCGCGCCu -3' miRNA: 3'- cacUGCGGUgcGCCGGUA------AUGCGCGG- -5' |
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31470 | 3' | -57.7 | NC_006650.1 | + | 14397 | 0.69 | 0.178016 |
Target: 5'- cGUGACaaaggauugcaucaGCCACGCGGCUuuagucaauccauaAUUgACGCGUg -3' miRNA: 3'- -CACUG--------------CGGUGCGCCGG--------------UAA-UGCGCGg -5' |
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31470 | 3' | -57.7 | NC_006650.1 | + | 17959 | 0.74 | 0.081141 |
Target: 5'- -gGGCuCCGCGUGGCCGUUucggggccuagGCGuCGCCg -3' miRNA: 3'- caCUGcGGUGCGCCGGUAA-----------UGC-GCGG- -5' |
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31470 | 3' | -57.7 | NC_006650.1 | + | 11438 | 0.74 | 0.081141 |
Target: 5'- -gGGCuCCGCGUGGCCGUUucggggccuagGCGuCGCCg -3' miRNA: 3'- caCUGcGGUGCGCCGGUAA-----------UGC-GCGG- -5' |
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31470 | 3' | -57.7 | NC_006650.1 | + | 14426 | 1.12 | 0.000075 |
Target: 5'- aGUGACGCCACGCGGCCAUUACGCGCCa -3' miRNA: 3'- -CACUGCGGUGCGCCGGUAAUGCGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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