Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31480 | 3' | -58.8 | NC_006653.1 | + | 20726 | 0.67 | 0.235685 |
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Target: 5'- cGUGCCaugaUGCCCUCGGCuuc-GCcucgGGCACa -3' miRNA: 3'- -UACGG----ACGGGGGCUGuaaaCG----CCGUG- -5' |
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| 31480 | 3' | -58.8 | NC_006653.1 | + | 5217 | 0.72 | 0.10206 |
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Target: 5'- gAUGCCUGCCCCCcccGACca--GCaGCACu -3' miRNA: 3'- -UACGGACGGGGG---CUGuaaaCGcCGUG- -5' |
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| 31480 | 3' | -58.8 | NC_006653.1 | + | 20567 | 0.73 | 0.090526 |
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Target: 5'- gAUGCCUGCCCCCccGACca--GCaGCACu -3' miRNA: 3'- -UACGGACGGGGG--CUGuaaaCGcCGUG- -5' |
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| 31480 | 3' | -58.8 | NC_006653.1 | + | 18863 | 0.73 | 0.090526 |
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Target: 5'- gAUGCCUGCCCCCccGACca--GCaGCACu -3' miRNA: 3'- -UACGGACGGGGG--CUGuaaaCGcCGUG- -5' |
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| 31480 | 3' | -58.8 | NC_006653.1 | + | 18774 | 1.08 | 0.000138 |
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Target: 5'- gAUGCCUGCCCCCGACAUUUGCGGCACg -3' miRNA: 3'- -UACGGACGGGGGCUGUAAACGCCGUG- -5' |
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| 31480 | 3' | -58.8 | NC_006653.1 | + | 20656 | 1.08 | 0.000138 |
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Target: 5'- gAUGCCUGCCCCCGACAUUUGCGGCACg -3' miRNA: 3'- -UACGGACGGGGGCUGUAAACGCCGUG- -5' |
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| 31480 | 3' | -58.8 | NC_006653.1 | + | 5307 | 1.08 | 0.000138 |
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Target: 5'- gAUGCCUGCCCCCGACAUUUGCGGCACg -3' miRNA: 3'- -UACGGACGGGGGCUGUAAACGCCGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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