Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31489 | 5' | -50.1 | NC_006655.1 | + | 11527 | 0.66 | 0.585203 |
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Target: 5'- ---aGAUcAGAUCUGGCCAuAUAUAAa -3' miRNA: 3'- uagaCUGcUCUAGACCGGUcUAUAUU- -5' |
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| 31489 | 5' | -50.1 | NC_006655.1 | + | 12263 | 0.66 | 0.572449 |
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Target: 5'- gAUCUGACcAGAuauaauuauaUCUGGUCAGAUcUAu -3' miRNA: 3'- -UAGACUGcUCU----------AGACCGGUCUAuAUu -5' |
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| 31489 | 5' | -50.1 | NC_006655.1 | + | 11201 | 0.67 | 0.485696 |
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Target: 5'- uAUCUGGCcAGAUCUcGUCAGAUAg-- -3' miRNA: 3'- -UAGACUGcUCUAGAcCGGUCUAUauu -5' |
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| 31489 | 5' | -50.1 | NC_006655.1 | + | 11128 | 0.67 | 0.47379 |
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Target: 5'- -aCgGAUuAGAUCUGGCCAGAUc--- -3' miRNA: 3'- uaGaCUGcUCUAGACCGGUCUAuauu -5' |
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| 31489 | 5' | -50.1 | NC_006655.1 | + | 11474 | 0.67 | 0.47379 |
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Target: 5'- gAUCUGGCcAGAUUuguuuauauaUGGCCAGAUcUGAu -3' miRNA: 3'- -UAGACUGcUCUAG----------ACCGGUCUAuAUU- -5' |
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| 31489 | 5' | -50.1 | NC_006655.1 | + | 11193 | 0.68 | 0.462033 |
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Target: 5'- gAUCUGACcAGAUCUGGUCAa------ -3' miRNA: 3'- -UAGACUGcUCUAGACCGGUcuauauu -5' |
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| 31489 | 5' | -50.1 | NC_006655.1 | + | 12215 | 0.68 | 0.462033 |
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Target: 5'- gAUCUGACcAGAuauaauuauaUCUGGUCAGAUcUAAa -3' miRNA: 3'- -UAGACUGcUCU----------AGACCGGUCUAuAUU- -5' |
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| 31489 | 5' | -50.1 | NC_006655.1 | + | 11231 | 0.74 | 0.18301 |
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Target: 5'- aAUUUGACcAGAUCUGGUCAGAUcUGAu -3' miRNA: 3'- -UAGACUGcUCUAGACCGGUCUAuAUU- -5' |
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| 31489 | 5' | -50.1 | NC_006655.1 | + | 11163 | 1.04 | 0.00102 |
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Target: 5'- uAUCUGACGAGAUCUGGCCAGAUAUAAu -3' miRNA: 3'- -UAGACUGCUCUAGACCGGUCUAUAUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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