miRNA: 3'- -CUGGGGUCUAGCCUcGAcUAuUAAGU- -5'
miRNA: 3'- -CUGGGGUCUAGcCUcGAcUAUUAAGU- -5'
miRNA: 3'- -CUGGGGUCUAGCCUcGAcUAuUAAGU- -5'
miRNA: 3'- cUGGGGUCUAGCCUcGAcUAUUAAGU- -5'
miRNA: 3'- -CUGGGGUCUAGCCUCGACUAUUAAGU- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31490 | 3' | -50.5 | NC_006655.1 | + | 13179 | 0.66 | 0.552599 |
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Target: 5'- gGACCCCAGGUUGGAcCUaAUcAUUUAg -3' miRNA: 3'- -CUGGGGUCUAGCCUcGAcUAuUAAGU- -5' |
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| 31490 | 3' | -50.5 | NC_006655.1 | + | 13146 | 0.67 | 0.515969 |
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Target: 5'- gGAUCCCAGGUCaGAcCUuAUAAUUCAg -3' miRNA: 3'- -CUGGGGUCUAGcCUcGAcUAUUAAGU- -5' |
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| 31490 | 3' | -50.5 | NC_006655.1 | + | 12784 | 0.71 | 0.297371 |
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Target: 5'- gGACCCCAGGUUGGAcCUcAUuAUUCAg -3' miRNA: 3'- -CUGGGGUCUAGCCUcGAcUAuUAAGU- -5' |
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| 31490 | 3' | -50.5 | NC_006655.1 | + | 13015 | 0.79 | 0.09437 |
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Target: 5'- uACCCCAGGUCGGAcCUcAUAAUUCAg -3' miRNA: 3'- cUGGGGUCUAGCCUcGAcUAUUAAGU- -5' |
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| 31490 | 3' | -50.5 | NC_006655.1 | + | 13047 | 1.09 | 0.000485 |
|
Target: 5'- gGACCCCAGAUCGGAGCUGAUAAUUCAg -3' miRNA: 3'- -CUGGGGUCUAGCCUCGACUAUUAAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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