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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31493 | 5' | -45.1 | NC_006657.1 | + | 10638 | 0.68 | 0.808294 |
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Target: 5'- -aUUAu--AUCUGGCCAGAUCUcGUc- -3' miRNA: 3'- uaAAUuucUAGACCGGUCUAGA-CAaa -5' |
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| 31493 | 5' | -45.1 | NC_006657.1 | + | 10599 | 0.74 | 0.466293 |
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Target: 5'- ----cGAGAUCUGGCCAGAuauaaucagaUCUGa-- -3' miRNA: 3'- uaaauUUCUAGACCGGUCU----------AGACaaa -5' |
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| 31493 | 5' | -45.1 | NC_006657.1 | + | 10558 | 0.78 | 0.307111 |
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Target: 5'- --aUAAacAGAUCUGGCCAGAUCUu--- -3' miRNA: 3'- uaaAUU--UCUAGACCGGUCUAGAcaaa -5' |
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| 31493 | 5' | -45.1 | NC_006657.1 | + | 10945 | 0.84 | 0.134591 |
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Target: 5'- --aUAAacAGAUCUGGCCAGAUCUGc-- -3' miRNA: 3'- uaaAUU--UCUAGACCGGUCUAGACaaa -5' |
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| 31493 | 5' | -45.1 | NC_006657.1 | + | 10664 | 0.84 | 0.125899 |
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Target: 5'- aAUUUGAccAGAUCUGGUCAGAUCUGa-- -3' miRNA: 3'- -UAAAUU--UCUAGACCGGUCUAGACaaa -5' |
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| 31493 | 5' | -45.1 | NC_006657.1 | + | 10900 | 0.94 | 0.028424 |
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Target: 5'- -cUUAGcAGAUCUGGCCAGAUCUGUUUa -3' miRNA: 3'- uaAAUU-UCUAGACCGGUCUAGACAAA- -5' |
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| 31493 | 5' | -45.1 | NC_006657.1 | + | 10605 | 1.05 | 0.004629 |
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Target: 5'- aAUUUAAAGAUCUGGCCAGAUCUGUUUa -3' miRNA: 3'- -UAAAUUUCUAGACCGGUCUAGACAAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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