Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31501 | 5' | -46.6 | NC_006658.1 | + | 25953 | 0.69 | 0.864233 |
Target: 5'- cCUAACaUACUCGCUGCGgaGAGcUGCu -3' miRNA: 3'- cGGUUG-AUGGGUGACGUaaCUUuACG- -5' |
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31501 | 5' | -46.6 | NC_006658.1 | + | 30239 | 0.69 | 0.845517 |
Target: 5'- cCCAGa-ACCCGCUggGCGUUGAcuaucAAUGCu -3' miRNA: 3'- cGGUUgaUGGGUGA--CGUAACU-----UUACG- -5' |
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31501 | 5' | -46.6 | NC_006658.1 | + | 25244 | 0.77 | 0.429108 |
Target: 5'- uGUCAGCaUGCCCugUGCuggcgaGAGGUGCa -3' miRNA: 3'- -CGGUUG-AUGGGugACGuaa---CUUUACG- -5' |
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31501 | 5' | -46.6 | NC_006658.1 | + | 28114 | 1.16 | 0.001319 |
Target: 5'- gGCCAACUACCCACUGCAUUGAAAUGCu -3' miRNA: 3'- -CGGUUGAUGGGUGACGUAACUUUACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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