Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31508 | 5' | -49.8 | NC_006659.1 | + | 23616 | 0.67 | 0.843679 |
Target: 5'- aCUUGUCaCCAAgUUGGcGGCAAGUc- -3' miRNA: 3'- -GAACGGcGGUUgAACCaCUGUUCAau -5' |
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31508 | 5' | -49.8 | NC_006659.1 | + | 27618 | 0.68 | 0.804244 |
Target: 5'- gCUUGCgGC--GCUUGGUGACucGUg- -3' miRNA: 3'- -GAACGgCGguUGAACCACUGuuCAau -5' |
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31508 | 5' | -49.8 | NC_006659.1 | + | 23721 | 0.68 | 0.761455 |
Target: 5'- aCUUGgCGaCAACUUGGUGAacaacCGAGUUc -3' miRNA: 3'- -GAACgGCgGUUGAACCACU-----GUUCAAu -5' |
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31508 | 5' | -49.8 | NC_006659.1 | + | 24134 | 0.7 | 0.668204 |
Target: 5'- --gGCCccugauaGCCAgagagaAUUUGGUGACAAGUUGc -3' miRNA: 3'- gaaCGG-------CGGU------UGAACCACUGUUCAAU- -5' |
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31508 | 5' | -49.8 | NC_006659.1 | + | 23772 | 0.76 | 0.35191 |
Target: 5'- aCUUGUCGUCAACUUGGUGcgaaaGAGUUu -3' miRNA: 3'- -GAACGGCGGUUGAACCACug---UUCAAu -5' |
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31508 | 5' | -49.8 | NC_006659.1 | + | 23680 | 1.08 | 0.002547 |
Target: 5'- aCUUGCCGCCAACUUGGUGACAAGUUAc -3' miRNA: 3'- -GAACGGCGGUUGAACCACUGUUCAAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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