Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31509 | 5' | -50.6 | NC_006659.1 | + | 34163 | 0.67 | 0.812787 |
Target: 5'- ----aUGGCGUGAGAC---AACGUGUc -3' miRNA: 3'- caugaACCGCGCUCUGaguUUGCACA- -5' |
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31509 | 5' | -50.6 | NC_006659.1 | + | 36298 | 0.67 | 0.792471 |
Target: 5'- uGUACUUGGCGCGAcAUUUuacaGUGa -3' miRNA: 3'- -CAUGAACCGCGCUcUGAGuuugCACa -5' |
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31509 | 5' | -50.6 | NC_006659.1 | + | 35125 | 0.67 | 0.792471 |
Target: 5'- uGUACUUGGCGCGAcAUUUuacaGUGa -3' miRNA: 3'- -CAUGAACCGCGCUcUGAGuuugCACa -5' |
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31509 | 5' | -50.6 | NC_006659.1 | + | 34193 | 0.88 | 0.054638 |
Target: 5'- uGUACUUGGCGCGAGACacuAACGUGUc -3' miRNA: 3'- -CAUGAACCGCGCUCUGaguUUGCACA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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