miRNA: 3'- -UAGACG---AUUcCgUCUAGAUCGGUCUA- -5'
miRNA: 3'- uagaCGauucCGUCUAGAUCGGUCUA- -5'
miRNA: 3'- -UAGACGauucCGUCUAGAUCGGUCUA- -5'
miRNA: 3'- -UAGACGAUUCCGUCUAGAUCGGUCUA- -5'
Results 1 - 4 of 4 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31517 | 3' | -52.6 | NC_006660.1 | + | 10618 | 0.67 | 0.39799 |
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Target: 5'- uGUCUGUaucUGAcG-AGAUCUGGCCAGAUa -3' miRNA: 3'- -UAGACG---AUUcCgUCUAGAUCGGUCUA- -5' |
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| 31517 | 3' | -52.6 | NC_006660.1 | + | 10922 | 0.68 | 0.33885 |
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Target: 5'- ---aGCcuuaGCAGAUCUGGCCAGAUc -3' miRNA: 3'- uagaCGauucCGUCUAGAUCGGUCUA- -5' |
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| 31517 | 3' | -52.6 | NC_006660.1 | + | 10619 | 0.8 | 0.048953 |
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Target: 5'- gAUCUGCcuuaGCAGAUCUGGCCAGAUc -3' miRNA: 3'- -UAGACGauucCGUCUAGAUCGGUCUA- -5' |
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| 31517 | 3' | -52.6 | NC_006660.1 | + | 10583 | 1.03 | 0.000764 |
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Target: 5'- gAUCUGCUAAGGCAGAUCUAGCCAGAUc -3' miRNA: 3'- -UAGACGAUUCCGUCUAGAUCGGUCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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