Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31517 | 5' | -50.1 | NC_006660.1 | + | 11621 | 0.67 | 0.612007 |
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Target: 5'- -gAUCUGACcAGAuauaauuguaUCUGGUCAGAUc- -3' miRNA: 3'- caUAGACUGcUCU----------AGACCGGUCUAua -5' |
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| 31517 | 5' | -50.1 | NC_006660.1 | + | 11673 | 0.67 | 0.612006 |
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Target: 5'- -gAUCUGACcAGAuacaauuauaUCUGGUCAGAUc- -3' miRNA: 3'- caUAGACUGcUCU----------AGACCGGUCUAua -5' |
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| 31517 | 5' | -50.1 | NC_006660.1 | + | 10654 | 0.69 | 0.490368 |
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Target: 5'- -gAUCUGACcAGAUCUGGUCAa---- -3' miRNA: 3'- caUAGACUGcUCUAGACCGGUcuaua -5' |
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| 31517 | 5' | -50.1 | NC_006660.1 | + | 10932 | 0.69 | 0.478763 |
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Target: 5'- -gAUCUGGCcAGAUCuguuuauauaUGGCCAGAUc- -3' miRNA: 3'- caUAGACUGcUCUAG----------ACCGGUCUAua -5' |
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| 31517 | 5' | -50.1 | NC_006660.1 | + | 10667 | 0.7 | 0.41222 |
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Target: 5'- aUAUCUGGCcAGAUCUcGUCAGAUAc -3' miRNA: 3'- cAUAGACUGcUCUAGAcCGGUCUAUa -5' |
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| 31517 | 5' | -50.1 | NC_006660.1 | + | 10629 | 0.72 | 0.323003 |
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Target: 5'- -gAUCUGGCuagaucugccuuaGcAGAUCUGGCCAGAUc- -3' miRNA: 3'- caUAGACUG-------------C-UCUAGACCGGUCUAua -5' |
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| 31517 | 5' | -50.1 | NC_006660.1 | + | 10553 | 0.73 | 0.306292 |
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Target: 5'- -gAUCUGGCcauauauaaacAGAUCUGGCCAGAUc- -3' miRNA: 3'- caUAGACUGc----------UCUAGACCGGUCUAua -5' |
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| 31517 | 5' | -50.1 | NC_006660.1 | + | 10984 | 0.73 | 0.306292 |
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Target: 5'- -gAUCUGGCcauauauaaacAGAUCUGGCCAGAUc- -3' miRNA: 3'- caUAGACUGc----------UCUAGACCGGUCUAua -5' |
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| 31517 | 5' | -50.1 | NC_006660.1 | + | 10696 | 0.74 | 0.265506 |
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Target: 5'- -aAUUUGACcAGAUCUGGUCAGAUc- -3' miRNA: 3'- caUAGACUGcUCUAGACCGGUCUAua -5' |
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| 31517 | 5' | -50.1 | NC_006660.1 | + | 10622 | 1.06 | 0.001134 |
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Target: 5'- uGUAUCUGACGAGAUCUGGCCAGAUAUa -3' miRNA: 3'- -CAUAGACUGCUCUAGACCGGUCUAUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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