Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31519 | 5' | -40.6 | NC_006661.1 | + | 11087 | 0.85 | 0.203318 |
Target: 5'- uCGGccAACAAUcACAUGUGAUCUGAGa -3' miRNA: 3'- -GUCauUUGUUAaUGUACACUAGACUC- -5' |
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31519 | 5' | -40.6 | NC_006661.1 | + | 6772 | 0.83 | 0.264835 |
Target: 5'- aGGUcAACAA--ACAUGUGGUCUGAGg -3' miRNA: 3'- gUCAuUUGUUaaUGUACACUAGACUC- -5' |
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31519 | 5' | -40.6 | NC_006661.1 | + | 7359 | 0.67 | 0.976216 |
Target: 5'- aCAGUccAGACuuUUcuaAUGUGGUCUGGGc -3' miRNA: 3'- -GUCA--UUUGuuAAug-UACACUAGACUC- -5' |
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31519 | 5' | -40.6 | NC_006661.1 | + | 11087 | 0.84 | 0.232368 |
Target: 5'- aGGUuGACAA--ACAUGUGGUCUGAGg -3' miRNA: 3'- gUCAuUUGUUaaUGUACACUAGACUC- -5' |
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31519 | 5' | -40.6 | NC_006661.1 | + | 6771 | 1.12 | 0.00381 |
Target: 5'- uCAGUAAACAAUUACAUGUGAUCUGAGa -3' miRNA: 3'- -GUCAUUUGUUAAUGUACACUAGACUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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