Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31523 | 3' | -49.2 | NC_006661.1 | + | 6733 | 0.71 | 0.2964 |
Target: 5'- -gUCUCAGAUCACAUGUaaUUGUUuACUg -3' miRNA: 3'- ugGGAGUCUGGUGUACA--AACAGuUGG- -5' |
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31523 | 3' | -49.2 | NC_006661.1 | + | 11126 | 0.8 | 0.071908 |
Target: 5'- -gUCUCAGAUCACAUGUgaUUGUUGGCCg -3' miRNA: 3'- ugGGAGUCUGGUGUACA--AACAGUUGG- -5' |
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31523 | 3' | -49.2 | NC_006661.1 | + | 6807 | 0.98 | 0.003602 |
Target: 5'- -gCCUCAGACCACAUGUUUGUUGACCu -3' miRNA: 3'- ugGGAGUCUGGUGUACAAACAGUUGG- -5' |
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31523 | 3' | -49.2 | NC_006661.1 | + | 11049 | 1.13 | 0.000266 |
Target: 5'- aACCCUCAGACCACAUGUUUGUCAACCu -3' miRNA: 3'- -UGGGAGUCUGGUGUACAAACAGUUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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