miRNA: 3'- agCCGGUUGUUgaUGUACACUAGACU- -5'
miRNA: 3'- agCCGGUUGUUgaUGUACACUAGACU- -5'
miRNA: 3'- -AGCCGG-----------UUGUUGAUGUACACUAGACU- -5'
miRNA: 3'- -AGCCGGUUGUUGaUGUACACUAGACU- -5'
Results 1 - 4 of 4 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31523 | 5' | -48.3 | NC_006661.1 | + | 11087 | 0.74 | 0.214963 |
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Target: 5'- -aGGUUGACAA--ACAUGUGGUCUGAg -3' miRNA: 3'- agCCGGUUGUUgaUGUACACUAGACU- -5' |
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| 31523 | 5' | -48.3 | NC_006661.1 | + | 6772 | 0.8 | 0.079829 |
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Target: 5'- -aGGUCAACAA--ACAUGUGGUCUGAg -3' miRNA: 3'- agCCGGUUGUUgaUGUACACUAGACU- -5' |
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| 31523 | 5' | -48.3 | NC_006661.1 | + | 6783 | 0.89 | 0.019147 |
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Target: 5'- cUCGGCUucgccucaguaAACAAUUACAUGUGAUCUGAg -3' miRNA: 3'- -AGCCGG-----------UUGUUGAUGUACACUAGACU- -5' |
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| 31523 | 5' | -48.3 | NC_006661.1 | + | 11086 | 0.99 | 0.003622 |
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Target: 5'- cUCGGCCAACAAUcACAUGUGAUCUGAg -3' miRNA: 3'- -AGCCGGUUGUUGaUGUACACUAGACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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