Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31525 | 5' | -50.1 | NC_006662.1 | + | 10788 | 0.67 | 0.528393 |
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Target: 5'- uAUCUGGCcAGAUCUcGUCAGAUAg-- -3' miRNA: 3'- -UAGACUGcUCUAGAcCGGUCUAUauu -5' |
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| 31525 | 5' | -50.1 | NC_006662.1 | + | 10166 | 0.67 | 0.528393 |
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Target: 5'- gAUCUGACcAGAuauaauuauaUCUGGUCAGAUcUAAu -3' miRNA: 3'- -UAGACUGcUCU----------AGACCGGUCUAuAUU- -5' |
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| 31525 | 5' | -50.1 | NC_006662.1 | + | 10862 | 0.67 | 0.516161 |
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Target: 5'- -aCgGAUuAGAUCUGGCCAGAUc--- -3' miRNA: 3'- uaGaCUGcUCUAGACCGGUCUAuauu -5' |
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| 31525 | 5' | -50.1 | NC_006662.1 | + | 10797 | 0.68 | 0.504045 |
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Target: 5'- gAUCUGACcAGAUCUGGUCAa------ -3' miRNA: 3'- -UAGACUGcUCUAGACCGGUcuauauu -5' |
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| 31525 | 5' | -50.1 | NC_006662.1 | + | 10525 | 0.68 | 0.492053 |
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Target: 5'- gAUCUGGCcAGAUCuguuuauauaUGGCCAGAUc--- -3' miRNA: 3'- -UAGACUGcUCUAG----------ACCGGUCUAuauu -5' |
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| 31525 | 5' | -50.1 | NC_006662.1 | + | 10236 | 0.68 | 0.468478 |
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Target: 5'- gAUCUGACcagauguaauuAGAUCUGaCCAGAUAUAAu -3' miRNA: 3'- -UAGACUGc----------UCUAGACcGGUCUAUAUU- -5' |
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| 31525 | 5' | -50.1 | NC_006662.1 | + | 10476 | 0.71 | 0.31324 |
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Target: 5'- gAUCUGGCcauauauaaacAGAUCUGGCCAGAUc--- -3' miRNA: 3'- -UAGACUGc----------UCUAGACCGGUCUAuauu -5' |
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| 31525 | 5' | -50.1 | NC_006662.1 | + | 10758 | 0.74 | 0.206498 |
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Target: 5'- aAUUUGACcAGAUCUGGUCAGAUcUGAu -3' miRNA: 3'- -UAGACUGcUCUAGACCGGUCUAuAUU- -5' |
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| 31525 | 5' | -50.1 | NC_006662.1 | + | 10827 | 1.04 | 0.001233 |
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Target: 5'- uAUCUGACGAGAUCUGGCCAGAUAUAAu -3' miRNA: 3'- -UAGACUGCUCUAGACCGGUCUAUAUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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