Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31528 | 3' | -46.4 | NC_006662.1 | + | 16551 | 0.66 | 0.829438 |
Target: 5'- ---aUCGUGUGCUUcgAUGGCAUUUu -3' miRNA: 3'- aaaaAGUAUACGGGaaUACCGUAGGc -5' |
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31528 | 3' | -46.4 | NC_006662.1 | + | 6155 | 0.73 | 0.457079 |
Target: 5'- uUUUUUUAUAcGCCUUUAUGGC-UCCc -3' miRNA: 3'- -AAAAAGUAUaCGGGAAUACCGuAGGc -5' |
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31528 | 3' | -46.4 | NC_006662.1 | + | 4533 | 0.78 | 0.244274 |
Target: 5'- aUUUUUAuucUAUGCCUUUAUGGCAUuuGu -3' miRNA: 3'- aAAAAGU---AUACGGGAAUACCGUAggC- -5' |
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31528 | 3' | -46.4 | NC_006662.1 | + | 6635 | 0.83 | 0.109057 |
Target: 5'- aUUUUUAUGauacGCCUUUAUGGCAUCCGa -3' miRNA: 3'- aAAAAGUAUa---CGGGAAUACCGUAGGC- -5' |
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31528 | 3' | -46.4 | NC_006662.1 | + | 6075 | 1.08 | 0.001762 |
Target: 5'- uUUUUUCAUAUGCCCUUAUGGCAUCCGa -3' miRNA: 3'- -AAAAAGUAUACGGGAAUACCGUAGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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