Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31530 | 3' | -47.4 | NC_006662.1 | + | 10797 | 0.68 | 0.701162 |
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Target: 5'- ------gAUCUGaCCAGAUCUgGUCAa -3' miRNA: 3'- acuaauaUAGACcGGUCUAGAgCAGU- -5' |
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| 31530 | 3' | -47.4 | NC_006662.1 | + | 10470 | 0.69 | 0.624969 |
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Target: 5'- aGAUUAgAUCUGGCCauauauaaacAGAUCUgGcCAg -3' miRNA: 3'- aCUAAUaUAGACCGG----------UCUAGAgCaGU- -5' |
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| 31530 | 3' | -47.4 | NC_006662.1 | + | 10525 | 0.69 | 0.624969 |
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Target: 5'- ------gAUCUGGCCAGAUCU-GUUu -3' miRNA: 3'- acuaauaUAGACCGGUCUAGAgCAGu -5' |
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| 31530 | 3' | -47.4 | NC_006662.1 | + | 10825 | 0.7 | 0.561533 |
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Target: 5'- ------gAUCUGGCCAGAUCUaaucCGUUu -3' miRNA: 3'- acuaauaUAGACCGGUCUAGA----GCAGu -5' |
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| 31530 | 3' | -47.4 | NC_006662.1 | + | 10181 | 0.75 | 0.318967 |
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Target: 5'- -aAUUAUAUCUGGUCAGAucuaauuacaUCUgGUCAg -3' miRNA: 3'- acUAAUAUAGACCGGUCU----------AGAgCAGU- -5' |
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| 31530 | 3' | -47.4 | NC_006662.1 | + | 10201 | 0.75 | 0.309983 |
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Target: 5'- -aAUUAUAUCUGGUCAGAUCUaaacCGUUu -3' miRNA: 3'- acUAAUAUAGACCGGUCUAGA----GCAGu -5' |
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| 31530 | 3' | -47.4 | NC_006662.1 | + | 10860 | 0.81 | 0.144252 |
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Target: 5'- gGAUUAgAUCUGGCCAGAUCUuuaaauUGUCu -3' miRNA: 3'- aCUAAUaUAGACCGGUCUAGA------GCAGu -5' |
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| 31530 | 3' | -47.4 | NC_006662.1 | + | 10781 | 1.1 | 0.001165 |
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Target: 5'- cUGAUUAUAUCUGGCCAGAUCUCGUCAg -3' miRNA: 3'- -ACUAAUAUAGACCGGUCUAGAGCAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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