Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31531 | 3' | -53.2 | NC_006820.1 | + | 104402 | 0.67 | 0.973081 |
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Target: 5'- aCAGcuCAUgCCCC-GUGGUgGGAGGAu -3' miRNA: 3'- cGUUuuGUGaGGGGaCACCA-CCUCCU- -5' |
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| 31531 | 3' | -53.2 | NC_006820.1 | + | 81643 | 0.67 | 0.970264 |
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Target: 5'- uCAGGA-ACUUCCCUGUGacgccUGGAGGGg -3' miRNA: 3'- cGUUUUgUGAGGGGACACc----ACCUCCU- -5' |
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| 31531 | 3' | -53.2 | NC_006820.1 | + | 175702 | 0.67 | 0.967241 |
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Target: 5'- uGCAucuccuGAugAgUUCUCUGUuuuaGGUGGAGGAa -3' miRNA: 3'- -CGU------UUugUgAGGGGACA----CCACCUCCU- -5' |
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| 31531 | 3' | -53.2 | NC_006820.1 | + | 5081 | 0.67 | 0.964008 |
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Target: 5'- uGUGAAcuggcACACU-CCCUGUuGUGGGGGGu -3' miRNA: 3'- -CGUUU-----UGUGAgGGGACAcCACCUCCU- -5' |
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| 31531 | 3' | -53.2 | NC_006820.1 | + | 190249 | 0.68 | 0.960557 |
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Target: 5'- aCAGAugGaaUCCUUUGUGG-GGAGGAa -3' miRNA: 3'- cGUUUugUg-AGGGGACACCaCCUCCU- -5' |
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| 31531 | 3' | -53.2 | NC_006820.1 | + | 58199 | 0.69 | 0.912109 |
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Target: 5'- uGCAucgguuauuuCAUUCCUCgGUGGUGGuGGAg -3' miRNA: 3'- -CGUuuu-------GUGAGGGGaCACCACCuCCU- -5' |
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| 31531 | 3' | -53.2 | NC_006820.1 | + | 11583 | 1.11 | 0.00482 |
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Target: 5'- gGCAAAACACUCCCCUGUGGUGGAGGAg -3' miRNA: 3'- -CGUUUUGUGAGGGGACACCACCUCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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