Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31531 | 5' | -44.5 | NC_006820.1 | + | 10988 | 0.66 | 0.999999 |
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Target: 5'- cGUCCUCAUUCAaaacuucuugaaUGA-GAGCAuuaggauauUCUUCu -3' miRNA: 3'- aCAGGAGUAAGU------------AUUaUUCGU---------AGGAG- -5' |
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| 31531 | 5' | -44.5 | NC_006820.1 | + | 71201 | 0.66 | 0.999999 |
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Target: 5'- cUGUUCUUGUggaacuUCAUGAUAAGUucuUCCUg -3' miRNA: 3'- -ACAGGAGUA------AGUAUUAUUCGu--AGGAg -5' |
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| 31531 | 5' | -44.5 | NC_006820.1 | + | 156082 | 0.67 | 0.999997 |
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Target: 5'- gGUCCUUuauaauuuggaucgUUAUAGUAuGCAUUCUCg -3' miRNA: 3'- aCAGGAGua------------AGUAUUAUuCGUAGGAG- -5' |
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| 31531 | 5' | -44.5 | NC_006820.1 | + | 36010 | 0.67 | 0.999994 |
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Target: 5'- gGUCUUCuAUUaaUAUAGUAAGCAuuUCCUa -3' miRNA: 3'- aCAGGAG-UAA--GUAUUAUUCGU--AGGAg -5' |
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| 31531 | 5' | -44.5 | NC_006820.1 | + | 93227 | 0.68 | 0.999992 |
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Target: 5'- -uUCUUUGUUCGUuuGAaAGGUAUCCUCg -3' miRNA: 3'- acAGGAGUAAGUA--UUaUUCGUAGGAG- -5' |
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| 31531 | 5' | -44.5 | NC_006820.1 | + | 105360 | 0.68 | 0.999992 |
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Target: 5'- -uUCCUuuagCAUUCAUcuucuuGUGAGCGUaCCUCa -3' miRNA: 3'- acAGGA----GUAAGUAu-----UAUUCGUA-GGAG- -5' |
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| 31531 | 5' | -44.5 | NC_006820.1 | + | 90010 | 0.69 | 0.999958 |
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Target: 5'- uUGUCUUCGUgaUCGUAAaacuGGUcgCCUCu -3' miRNA: 3'- -ACAGGAGUA--AGUAUUau--UCGuaGGAG- -5' |
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| 31531 | 5' | -44.5 | NC_006820.1 | + | 81414 | 0.69 | 0.999958 |
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Target: 5'- aGUCCUCAUccuccguuUCGUAuaagGGGCAugguUCUUCa -3' miRNA: 3'- aCAGGAGUA--------AGUAUua--UUCGU----AGGAG- -5' |
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| 31531 | 5' | -44.5 | NC_006820.1 | + | 11539 | 1.11 | 0.03441 |
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Target: 5'- gUGUCCUCAUUCAUAAUAAGCAUCCUCc -3' miRNA: 3'- -ACAGGAGUAAGUAUUAUUCGUAGGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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