Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31535 | 3' | -58.8 | NC_006820.1 | + | 23580 | 0.67 | 0.767773 |
Target: 5'- --cCCCUUGGcCgAUGCAGCGGGCu-- -3' miRNA: 3'- uacGGGAGCCaG-UACGUCGCCUGcuu -5' |
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31535 | 3' | -58.8 | NC_006820.1 | + | 183265 | 0.68 | 0.690377 |
Target: 5'- -gGUCCUgGGggCGgcgGCGGCGGugGAGg -3' miRNA: 3'- uaCGGGAgCCa-GUa--CGUCGCCugCUU- -5' |
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31535 | 3' | -58.8 | NC_006820.1 | + | 183292 | 0.68 | 0.690377 |
Target: 5'- gGUGCCCcUGG-CGgcgGCGGUGGugGAGu -3' miRNA: 3'- -UACGGGaGCCaGUa--CGUCGCCugCUU- -5' |
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31535 | 3' | -58.8 | NC_006820.1 | + | 24134 | 1.03 | 0.003897 |
Target: 5'- cAUGCCCUCGGUCAUGCAGCGGACGAAc -3' miRNA: 3'- -UACGGGAGCCAGUACGUCGCCUGCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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