Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31536 | 3' | -47.1 | NC_006820.1 | + | 22991 | 0.66 | 0.999971 |
Target: 5'- uGCAGaAUGaaUAGAAAAGGgGgGAGGGUu -3' miRNA: 3'- gUGUC-UAC--GUCUUUUUCgCaUUCCCG- -5' |
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31536 | 3' | -47.1 | NC_006820.1 | + | 26206 | 0.66 | 0.999971 |
Target: 5'- --uGGAUuaAGAAuauuucaacAAAGCGUGAGGaGCu -3' miRNA: 3'- gugUCUAcgUCUU---------UUUCGCAUUCC-CG- -5' |
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31536 | 3' | -47.1 | NC_006820.1 | + | 161338 | 0.66 | 0.999961 |
Target: 5'- -cUAGAUaGUAauGAGcuuGAGCGUAAGGGUu -3' miRNA: 3'- guGUCUA-CGU--CUUu--UUCGCAUUCCCG- -5' |
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31536 | 3' | -47.1 | NC_006820.1 | + | 80206 | 0.66 | 0.999941 |
Target: 5'- uCAUAGGUGcCAGGAGcAGCGUcguuaagaagagcAGGGUu -3' miRNA: 3'- -GUGUCUAC-GUCUUUuUCGCAu------------UCCCG- -5' |
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31536 | 3' | -47.1 | NC_006820.1 | + | 152197 | 0.66 | 0.99993 |
Target: 5'- gCACAGAgaaaAGAAAAGGCG-AAGGa- -3' miRNA: 3'- -GUGUCUacg-UCUUUUUCGCaUUCCcg -5' |
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31536 | 3' | -47.1 | NC_006820.1 | + | 135838 | 0.67 | 0.999798 |
Target: 5'- aACAGggGCuGAGuAAGCGacacaaauccAAGGGCg -3' miRNA: 3'- gUGUCuaCGuCUUuUUCGCa---------UUCCCG- -5' |
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31536 | 3' | -47.1 | NC_006820.1 | + | 145710 | 0.67 | 0.999671 |
Target: 5'- gGCAGAcagcUGaGGAAGAGGCacaGGGGGCu -3' miRNA: 3'- gUGUCU----ACgUCUUUUUCGca-UUCCCG- -5' |
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31536 | 3' | -47.1 | NC_006820.1 | + | 5378 | 0.68 | 0.999585 |
Target: 5'- -uCAGGaucUGCuGGAGAcgGGCGUucauGGGGCg -3' miRNA: 3'- guGUCU---ACGuCUUUU--UCGCAu---UCCCG- -5' |
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31536 | 3' | -47.1 | NC_006820.1 | + | 37129 | 0.68 | 0.99948 |
Target: 5'- gGCGGugcuaggGCAGAAAucuuAGCGguugcuGGGCa -3' miRNA: 3'- gUGUCua-----CGUCUUUu---UCGCauu---CCCG- -5' |
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31536 | 3' | -47.1 | NC_006820.1 | + | 108961 | 0.68 | 0.99948 |
Target: 5'- gGCAGAUGCAGuAAucGUG-AAGGuaGCa -3' miRNA: 3'- gUGUCUACGUCuUUuuCGCaUUCC--CG- -5' |
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31536 | 3' | -47.1 | NC_006820.1 | + | 120052 | 0.68 | 0.9992 |
Target: 5'- cCACGGAUGCucguAAGGUu--GGGGCa -3' miRNA: 3'- -GUGUCUACGucuuUUUCGcauUCCCG- -5' |
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31536 | 3' | -47.1 | NC_006820.1 | + | 111512 | 0.68 | 0.999183 |
Target: 5'- aCACaAGAUGguuGAAGAGGCGUGugaggaccuugcaGGGGUc -3' miRNA: 3'- -GUG-UCUACgu-CUUUUUCGCAU-------------UCCCG- -5' |
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31536 | 3' | -47.1 | NC_006820.1 | + | 123703 | 0.69 | 0.998236 |
Target: 5'- aGCAGGagUGCcGAcAAAGUugacGUAGGGGCg -3' miRNA: 3'- gUGUCU--ACGuCUuUUUCG----CAUUCCCG- -5' |
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31536 | 3' | -47.1 | NC_006820.1 | + | 80021 | 0.69 | 0.99788 |
Target: 5'- --aAGAUGCcucguGAAGaucuuGAGCGUAuGGGCg -3' miRNA: 3'- gugUCUACGu----CUUU-----UUCGCAUuCCCG- -5' |
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31536 | 3' | -47.1 | NC_006820.1 | + | 183121 | 0.7 | 0.996436 |
Target: 5'- gGCGGcgGUGGAGGAGGUG-GAGGaGCu -3' miRNA: 3'- gUGUCuaCGUCUUUUUCGCaUUCC-CG- -5' |
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31536 | 3' | -47.1 | NC_006820.1 | + | 85589 | 0.72 | 0.986789 |
Target: 5'- gUACAGAUGCcGAAAucGUcauGGGGCa -3' miRNA: 3'- -GUGUCUACGuCUUUuuCGcauUCCCG- -5' |
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31536 | 3' | -47.1 | NC_006820.1 | + | 74841 | 0.73 | 0.976137 |
Target: 5'- -uCGaAUGCAGAAGGAGCaGcAAGGGCa -3' miRNA: 3'- guGUcUACGUCUUUUUCG-CaUUCCCG- -5' |
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31536 | 3' | -47.1 | NC_006820.1 | + | 154583 | 0.73 | 0.976137 |
Target: 5'- cUACAGAcgcUGCuGGAGGGGCagcgAGGGGCg -3' miRNA: 3'- -GUGUCU---ACGuCUUUUUCGca--UUCCCG- -5' |
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31536 | 3' | -47.1 | NC_006820.1 | + | 26480 | 1.14 | 0.012401 |
Target: 5'- gCACAGAUGCAGAAAAAGCGUAAGGGCa -3' miRNA: 3'- -GUGUCUACGUCUUUUUCGCAUUCCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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