miRNA: 3'- guugACUAUc-UAAUGAAGGUCACGGU- -5'
miRNA: 3'- -GUUGACUAUCUAAUG---AAGGuCA-CGGU- -5'
miRNA: 3'- -GUUGACuaUCUAAUgaaGGUCACGGU- -5'
miRNA: 3'- gUUgACUAUCUaa-UGA--------AGGUCACGGU- -5'
miRNA: 3'- -GUUGACUAUCUAAUGAAGGUCACGGU- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31538 | 3' | -46.3 | NC_006820.1 | + | 43500 | 0.66 | 0.999987 |
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Target: 5'- ---aUGAUGuaGUUACUUCUAGUGUCGu -3' miRNA: 3'- guugACUAUc-UAAUGAAGGUCACGGU- -5' |
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| 31538 | 3' | -46.3 | NC_006820.1 | + | 185545 | 0.66 | 0.999966 |
|
Target: 5'- uUAACUGAUGGAUUAUuugUUCCuGUcGUUAu -3' miRNA: 3'- -GUUGACUAUCUAAUG---AAGGuCA-CGGU- -5' |
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| 31538 | 3' | -46.3 | NC_006820.1 | + | 80757 | 0.7 | 0.997983 |
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Target: 5'- cCAACUGcgAGGUUA---CCAGUGUCAu -3' miRNA: 3'- -GUUGACuaUCUAAUgaaGGUCACGGU- -5' |
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| 31538 | 3' | -46.3 | NC_006820.1 | + | 40264 | 0.7 | 0.997582 |
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Target: 5'- gAGgUGGUAGAacaACUucuagugcUCCAGUGCCAg -3' miRNA: 3'- gUUgACUAUCUaa-UGA--------AGGUCACGGU- -5' |
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| 31538 | 3' | -46.3 | NC_006820.1 | + | 62329 | 1.1 | 0.026892 |
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Target: 5'- cCAACUGAUAGAUUACUUCCAGUGCCAc -3' miRNA: 3'- -GUUGACUAUCUAAUGAAGGUCACGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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