Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31539 | 3' | -47.3 | NC_006820.1 | + | 72221 | 0.67 | 0.999877 |
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Target: 5'- uGGUUag-UUCAGCAAGAuuACUUCAa- -3' miRNA: 3'- gCCAAcuaGAGUCGUUCU--UGAAGUga -5' |
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| 31539 | 3' | -47.3 | NC_006820.1 | + | 80176 | 0.69 | 0.998796 |
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Target: 5'- uGGUcuUGAUgCUgAGCAAGAGCUU-GCUa -3' miRNA: 3'- gCCA--ACUA-GAgUCGUUCUUGAAgUGA- -5' |
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| 31539 | 3' | -47.3 | NC_006820.1 | + | 60113 | 0.69 | 0.998796 |
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Target: 5'- -aGUUGAUUacaUCAGCuGGAACaUCACUa -3' miRNA: 3'- gcCAACUAG---AGUCGuUCUUGaAGUGA- -5' |
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| 31539 | 3' | -47.3 | NC_006820.1 | + | 34879 | 0.69 | 0.997883 |
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Target: 5'- uGGUUGGUCUCuGCGAGGuuggCAUUu -3' miRNA: 3'- gCCAACUAGAGuCGUUCUugaaGUGA- -5' |
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| 31539 | 3' | -47.3 | NC_006820.1 | + | 79750 | 0.7 | 0.99645 |
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Target: 5'- ----cGGUCUCcaauuGCAGGAGCUUCGCc -3' miRNA: 3'- gccaaCUAGAGu----CGUUCUUGAAGUGa -5' |
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| 31539 | 3' | -47.3 | NC_006820.1 | + | 134472 | 0.72 | 0.980939 |
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Target: 5'- aGGggGAacuggcgagaacuUCUUAGCAGGuGACUUCACUc -3' miRNA: 3'- gCCaaCU-------------AGAGUCGUUC-UUGAAGUGA- -5' |
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| 31539 | 3' | -47.3 | NC_006820.1 | + | 80438 | 1.09 | 0.026433 |
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Target: 5'- gCGGUUGAUCUCAGCAAGAACUUCACUc -3' miRNA: 3'- -GCCAACUAGAGUCGUUCUUGAAGUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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