Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31539 | 5' | -50.5 | NC_006820.1 | + | 46345 | 0.66 | 0.994264 |
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Target: 5'- cUUGGUuuuuGCUCUUGCUCuGUAUUAu- -3' miRNA: 3'- -AAUCGuu--CGAGAACGAGuCGUAGUcu -5' |
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| 31539 | 5' | -50.5 | NC_006820.1 | + | 144622 | 0.66 | 0.992299 |
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Target: 5'- -aAGCAAGCuggUCUUGCUaaguGGCGUaAGAg -3' miRNA: 3'- aaUCGUUCG---AGAACGAg---UCGUAgUCU- -5' |
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| 31539 | 5' | -50.5 | NC_006820.1 | + | 155680 | 0.66 | 0.992299 |
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Target: 5'- aUAuCGAGUUCUUcucuagcacGCUCAGCAUCAu- -3' miRNA: 3'- aAUcGUUCGAGAA---------CGAGUCGUAGUcu -5' |
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| 31539 | 5' | -50.5 | NC_006820.1 | + | 69643 | 0.66 | 0.991134 |
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Target: 5'- cUAGCGccuccuGGCcCUUGCUCAGCA--AGGc -3' miRNA: 3'- aAUCGU------UCGaGAACGAGUCGUagUCU- -5' |
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| 31539 | 5' | -50.5 | NC_006820.1 | + | 193714 | 0.67 | 0.988389 |
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Target: 5'- -cAGCAAcGCUCaUGCUCAGUcuuguuAUCAuGAg -3' miRNA: 3'- aaUCGUU-CGAGaACGAGUCG------UAGU-CU- -5' |
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| 31539 | 5' | -50.5 | NC_006820.1 | + | 78533 | 0.68 | 0.976137 |
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Target: 5'- gUUGGCAuaGGaUCUUGC-CAGCcUCAGAa -3' miRNA: 3'- -AAUCGU--UCgAGAACGaGUCGuAGUCU- -5' |
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| 31539 | 5' | -50.5 | NC_006820.1 | + | 107106 | 0.69 | 0.952205 |
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Target: 5'- --uGCAGGgUCUggaGCUcCAGCAUUAGAu -3' miRNA: 3'- aauCGUUCgAGAa--CGA-GUCGUAGUCU- -5' |
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| 31539 | 5' | -50.5 | NC_006820.1 | + | 461 | 0.7 | 0.947805 |
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Target: 5'- -gAGCAuaCUCUUGCUUgucuGGCGUUAGAg -3' miRNA: 3'- aaUCGUucGAGAACGAG----UCGUAGUCU- -5' |
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| 31539 | 5' | -50.5 | NC_006820.1 | + | 154493 | 0.7 | 0.92758 |
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Target: 5'- -cAGCAGGCUCcaaUGC-CAGCGaugguUCAGAu -3' miRNA: 3'- aaUCGUUCGAGa--ACGaGUCGU-----AGUCU- -5' |
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| 31539 | 5' | -50.5 | NC_006820.1 | + | 80402 | 0.99 | 0.035309 |
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Target: 5'- gUUAGCAAGCUCUUGCUCAGCAUCAaGAc -3' miRNA: 3'- -AAUCGUUCGAGAACGAGUCGUAGU-CU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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