Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31540 | 3' | -45.8 | NC_006820.1 | + | 145979 | 0.66 | 0.999993 |
Target: 5'- -----cCUGCUCCCgaaGGAGAGUcucUCCc -3' miRNA: 3'- uuuuuaGAUGGGGGag-UUUCUCA---AGG- -5' |
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31540 | 3' | -45.8 | NC_006820.1 | + | 79114 | 0.67 | 0.999981 |
Target: 5'- ---cAUUgACCUCCUCAGcaAGUUCCu -3' miRNA: 3'- uuuuUAGaUGGGGGAGUUucUCAAGG- -5' |
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31540 | 3' | -45.8 | NC_006820.1 | + | 23918 | 0.67 | 0.999981 |
Target: 5'- gGGGGAUCUcgguCCCCCUUguGGcAGUUCa -3' miRNA: 3'- -UUUUUAGAu---GGGGGAGuuUC-UCAAGg -5' |
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31540 | 3' | -45.8 | NC_006820.1 | + | 24611 | 0.67 | 0.999974 |
Target: 5'- ----cUCUGCCUUCUCAGcggcggcguAGAG-UCCg -3' miRNA: 3'- uuuuuAGAUGGGGGAGUU---------UCUCaAGG- -5' |
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31540 | 3' | -45.8 | NC_006820.1 | + | 5253 | 0.67 | 0.999964 |
Target: 5'- gAAGGcgCUcaACCgCUUCcAAGAGUUCCu -3' miRNA: 3'- -UUUUuaGA--UGGgGGAGuUUCUCAAGG- -5' |
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31540 | 3' | -45.8 | NC_006820.1 | + | 11561 | 0.67 | 0.999964 |
Target: 5'- gAGGAGUgUGCCagUUCAAGGAGUgUCCu -3' miRNA: 3'- -UUUUUAgAUGGggGAGUUUCUCA-AGG- -5' |
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31540 | 3' | -45.8 | NC_006820.1 | + | 5637 | 0.67 | 0.999952 |
Target: 5'- gGAAuAUCUGCUCCCUgguauuggUGAAGAGUaucauuaucucuUCCg -3' miRNA: 3'- -UUUuUAGAUGGGGGA--------GUUUCUCA------------AGG- -5' |
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31540 | 3' | -45.8 | NC_006820.1 | + | 112873 | 0.68 | 0.999942 |
Target: 5'- ----cUCUGCUCUCUCuuucagaagauccaaAAAGAGUUCUu -3' miRNA: 3'- uuuuuAGAUGGGGGAG---------------UUUCUCAAGG- -5' |
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31540 | 3' | -45.8 | NC_006820.1 | + | 130871 | 0.68 | 0.999935 |
Target: 5'- -----cUUACCCCaaCcAGGAGUUCCa -3' miRNA: 3'- uuuuuaGAUGGGGgaGuUUCUCAAGG- -5' |
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31540 | 3' | -45.8 | NC_006820.1 | + | 16723 | 0.68 | 0.999935 |
Target: 5'- ---uAUCUACCCCCgaugaccaguUUGAAGAGUgUCa -3' miRNA: 3'- uuuuUAGAUGGGGG----------AGUUUCUCAaGG- -5' |
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31540 | 3' | -45.8 | NC_006820.1 | + | 90035 | 0.68 | 0.999887 |
Target: 5'- -------gAUCCCUUCAuugagGGGAGUUCCa -3' miRNA: 3'- uuuuuagaUGGGGGAGU-----UUCUCAAGG- -5' |
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31540 | 3' | -45.8 | NC_006820.1 | + | 135666 | 0.69 | 0.999755 |
Target: 5'- --uGGUCUACCCCUUUGGAcaaGGUUCUu -3' miRNA: 3'- uuuUUAGAUGGGGGAGUUUc--UCAAGG- -5' |
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31540 | 3' | -45.8 | NC_006820.1 | + | 7644 | 0.77 | 0.94252 |
Target: 5'- ---uGUCUGCCUCCUCAcAGGAuUUCCa -3' miRNA: 3'- uuuuUAGAUGGGGGAGU-UUCUcAAGG- -5' |
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31540 | 3' | -45.8 | NC_006820.1 | + | 81778 | 1.12 | 0.02172 |
Target: 5'- aAAAAAUCUACCCCCUCAAAGAGUUCCa -3' miRNA: 3'- -UUUUUAGAUGGGGGAGUUUCUCAAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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