Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31542 | 3' | -50.4 | NC_006820.1 | + | 122655 | 0.66 | 0.998239 |
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Target: 5'- -cCCUGgucgguuuGAAGGUUCCCCUUCUCccgagucuUGGAa -3' miRNA: 3'- cuGGAU--------UUUUUAGGGGGAGGAG--------ACCU- -5' |
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| 31542 | 3' | -50.4 | NC_006820.1 | + | 155940 | 0.66 | 0.996503 |
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Target: 5'- uACCUAAuc--UCCCCaaUCCUUUGGGc -3' miRNA: 3'- cUGGAUUuuuuAGGGGg-AGGAGACCU- -5' |
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| 31542 | 3' | -50.4 | NC_006820.1 | + | 21004 | 0.67 | 0.992565 |
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Target: 5'- --aCUGAuuAAGAUUCCCUUCUucUCUGGAa -3' miRNA: 3'- cugGAUU--UUUUAGGGGGAGG--AGACCU- -5' |
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| 31542 | 3' | -50.4 | NC_006820.1 | + | 113941 | 0.7 | 0.969086 |
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Target: 5'- aGACCUGAgGAGGUCUCCCUCCa----- -3' miRNA: 3'- -CUGGAUU-UUUUAGGGGGAGGagaccu -5' |
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| 31542 | 3' | -50.4 | NC_006820.1 | + | 23709 | 0.73 | 0.895952 |
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Target: 5'- gGACC---GAGAUCCCCCUCCcCUGc- -3' miRNA: 3'- -CUGGauuUUUUAGGGGGAGGaGACcu -5' |
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| 31542 | 3' | -50.4 | NC_006820.1 | + | 116913 | 1.09 | 0.011278 |
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Target: 5'- aGACCUAAAAAAUCCCCCUCCUCUGGAc -3' miRNA: 3'- -CUGGAUUUUUUAGGGGGAGGAGACCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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