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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31544 | 3' | -52.6 | NC_006820.1 | + | 66142 | 0.67 | 0.967305 |
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Target: 5'- -aUGUACCAccaauauuuacUCCUCCUCUCCAUUg- -3' miRNA: 3'- uaAUAUGGUc----------AGGAGGAGGGGUAGug -5' |
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| 31544 | 3' | -52.6 | NC_006820.1 | + | 68810 | 0.68 | 0.948553 |
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Target: 5'- -aUAUGCUAGaggaacuuuuUCUUCCUCCaguaCAUCACc -3' miRNA: 3'- uaAUAUGGUC----------AGGAGGAGGg---GUAGUG- -5' |
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| 31544 | 3' | -52.6 | NC_006820.1 | + | 24665 | 0.69 | 0.939381 |
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Target: 5'- ----cACCAGaCCauggggcaggaCCUCCCCAUCACc -3' miRNA: 3'- uaauaUGGUCaGGa----------GGAGGGGUAGUG- -5' |
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| 31544 | 3' | -52.6 | NC_006820.1 | + | 58074 | 0.72 | 0.82247 |
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Target: 5'- --aGUACCAGUCUUCCaauUUCUCAUUGCa -3' miRNA: 3'- uaaUAUGGUCAGGAGG---AGGGGUAGUG- -5' |
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| 31544 | 3' | -52.6 | NC_006820.1 | + | 120839 | 1.07 | 0.008206 |
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Target: 5'- uAUUAUACCAGUCCUCCUCCCCAUCACu -3' miRNA: 3'- -UAAUAUGGUCAGGAGGAGGGGUAGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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