Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31545 | 3' | -55.9 | NC_006820.1 | + | 175772 | 0.66 | 0.942034 |
Target: 5'- uGCC-CCACCAcCACCAACUgaACUAu -3' miRNA: 3'- gUGGcGGUGGUcGUGGUUGAacUGGU- -5' |
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31545 | 3' | -55.9 | NC_006820.1 | + | 156868 | 0.66 | 0.9273 |
Target: 5'- uCACCaucaaCACCAGCACCuaguGCUccGCCAa -3' miRNA: 3'- -GUGGcg---GUGGUCGUGGu---UGAacUGGU- -5' |
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31545 | 3' | -55.9 | NC_006820.1 | + | 177129 | 0.67 | 0.904364 |
Target: 5'- aGCUGCCAUCGGCAC-AAUUauGCCAu -3' miRNA: 3'- gUGGCGGUGGUCGUGgUUGAacUGGU- -5' |
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31545 | 3' | -55.9 | NC_006820.1 | + | 156483 | 0.67 | 0.891505 |
Target: 5'- cCACCGaaagauCCACCcuucaucguggcAGCACUAGCagGACCAc -3' miRNA: 3'- -GUGGC------GGUGG------------UCGUGGUUGaaCUGGU- -5' |
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31545 | 3' | -55.9 | NC_006820.1 | + | 23600 | 0.68 | 0.86313 |
Target: 5'- aGgCGUCACgAGCACCuguccccCUUGGCCGa -3' miRNA: 3'- gUgGCGGUGgUCGUGGuu-----GAACUGGU- -5' |
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31545 | 3' | -55.9 | NC_006820.1 | + | 132704 | 0.68 | 0.839677 |
Target: 5'- uGCU-CCACCAGCACCAACagGuaauaACCAa -3' miRNA: 3'- gUGGcGGUGGUCGUGGUUGaaC-----UGGU- -5' |
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31545 | 3' | -55.9 | NC_006820.1 | + | 21686 | 0.69 | 0.831479 |
Target: 5'- cUugUGCCACUGGC-CCGACc-GACCAc -3' miRNA: 3'- -GugGCGGUGGUCGuGGUUGaaCUGGU- -5' |
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31545 | 3' | -55.9 | NC_006820.1 | + | 63215 | 0.7 | 0.778809 |
Target: 5'- aUACCGauguuCCAaUACCAACUUGACCAu -3' miRNA: 3'- -GUGGCggu--GGUcGUGGUUGAACUGGU- -5' |
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31545 | 3' | -55.9 | NC_006820.1 | + | 80727 | 0.7 | 0.769528 |
Target: 5'- uCACCGauggaaggaCCACCAGCGCCGuUUaGACCu -3' miRNA: 3'- -GUGGC---------GGUGGUCGUGGUuGAaCUGGu -5' |
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31545 | 3' | -55.9 | NC_006820.1 | + | 183323 | 0.71 | 0.728355 |
Target: 5'- cCGCCGCCGCCAGCaacauuacaucggaACCAGCaaGAg-- -3' miRNA: 3'- -GUGGCGGUGGUCG--------------UGGUUGaaCUggu -5' |
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31545 | 3' | -55.9 | NC_006820.1 | + | 143833 | 0.71 | 0.681645 |
Target: 5'- uCGCCGacuucuccgacuCCACCAaCACCAGCUUG-CCAa -3' miRNA: 3'- -GUGGC------------GGUGGUcGUGGUUGAACuGGU- -5' |
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31545 | 3' | -55.9 | NC_006820.1 | + | 183485 | 0.73 | 0.610681 |
Target: 5'- cCACCGCCGCCGcCGCCcccagGACCAu -3' miRNA: 3'- -GUGGCGGUGGUcGUGGuugaaCUGGU- -5' |
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31545 | 3' | -55.9 | NC_006820.1 | + | 183554 | 0.78 | 0.322779 |
Target: 5'- cCACCGCCuccuCCAGCGCCGccagagcaguCUUGACCu -3' miRNA: 3'- -GUGGCGGu---GGUCGUGGUu---------GAACUGGu -5' |
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31545 | 3' | -55.9 | NC_006820.1 | + | 183270 | 0.79 | 0.308443 |
Target: 5'- gCACCuCCACCAGCACCAcaACcaGACCAu -3' miRNA: 3'- -GUGGcGGUGGUCGUGGU--UGaaCUGGU- -5' |
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31545 | 3' | -55.9 | NC_006820.1 | + | 123729 | 1.08 | 0.003755 |
Target: 5'- cCACCGCCACCAGCACCAACUUGACCAg -3' miRNA: 3'- -GUGGCGGUGGUCGUGGUUGAACUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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