Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31546 | 5' | -45.2 | NC_006820.1 | + | 154289 | 0.66 | 0.999997 |
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Target: 5'- cCUAAGAGAguUGGCGCcgCCGAaucaGCAu -3' miRNA: 3'- -GAUUCUUUauACUGCGa-GGUUga--CGU- -5' |
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| 31546 | 5' | -45.2 | NC_006820.1 | + | 59882 | 0.67 | 0.999986 |
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Target: 5'- --uAGAAucuaGUGAUGUUCCAGCUGa- -3' miRNA: 3'- gauUCUUua--UACUGCGAGGUUGACgu -5' |
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| 31546 | 5' | -45.2 | NC_006820.1 | + | 103066 | 0.67 | 0.999981 |
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Target: 5'- aCUAAGugggAUGGUGCUCCuGCUGUg -3' miRNA: 3'- -GAUUCuuuaUACUGCGAGGuUGACGu -5' |
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| 31546 | 5' | -45.2 | NC_006820.1 | + | 17171 | 0.68 | 0.999951 |
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Target: 5'- uCUGAuGcuGUGUGAUGCUCUccagcaGACUGCu -3' miRNA: 3'- -GAUU-CuuUAUACUGCGAGG------UUGACGu -5' |
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| 31546 | 5' | -45.2 | NC_006820.1 | + | 179641 | 0.69 | 0.999885 |
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Target: 5'- --uGGuAGUAUGACGCUUgCGACUGUu -3' miRNA: 3'- gauUCuUUAUACUGCGAG-GUUGACGu -5' |
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| 31546 | 5' | -45.2 | NC_006820.1 | + | 152076 | 0.77 | 0.954734 |
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Target: 5'- -cAAGAAGUAUGGCGgUCCAAaUGCu -3' miRNA: 3'- gaUUCUUUAUACUGCgAGGUUgACGu -5' |
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| 31546 | 5' | -45.2 | NC_006820.1 | + | 152265 | 1.1 | 0.03347 |
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Target: 5'- uCUAAGAAAUAUGACGCUCCAACUGCAu -3' miRNA: 3'- -GAUUCUUUAUACUGCGAGGUUGACGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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