Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31547 | 5' | -46.9 | NC_006820.1 | + | 103675 | 0.66 | 0.999969 |
Target: 5'- aGCGACUaagcaguuUACGCUUgguuaUACGauGAAGCGUUu -3' miRNA: 3'- cUGCUGG--------AUGCGAA-----AUGU--UUUCGCAA- -5' |
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31547 | 5' | -46.9 | NC_006820.1 | + | 47258 | 0.66 | 0.999926 |
Target: 5'- --aGACCUAUGCgUUUGCAAAuGCa-- -3' miRNA: 3'- cugCUGGAUGCG-AAAUGUUUuCGcaa -5' |
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31547 | 5' | -46.9 | NC_006820.1 | + | 146050 | 0.7 | 0.996824 |
Target: 5'- uGCGACUUGCGCUUaagaucUGCAAAAGacauGUUg -3' miRNA: 3'- cUGCUGGAUGCGAA------AUGUUUUCg---CAA- -5' |
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31547 | 5' | -46.9 | NC_006820.1 | + | 164557 | 1.07 | 0.031895 |
Target: 5'- cGACGACCUACGCUUUACAAAAGCGUUg -3' miRNA: 3'- -CUGCUGGAUGCGAAAUGUUUUCGCAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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