Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31550 | 3' | -53.8 | NC_006820.1 | + | 123396 | 0.67 | 0.943254 |
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Target: 5'- aGAa-CCGcACUGUUGCuGGUGGCACc -3' miRNA: 3'- -CUgcGGUuUGACGACGuCUACCGUGu -5' |
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| 31550 | 3' | -53.8 | NC_006820.1 | + | 39732 | 0.67 | 0.938519 |
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Target: 5'- aACGgaAucAAUUGCUGUAGAUGGUGCAa -3' miRNA: 3'- cUGCggU--UUGACGACGUCUACCGUGU- -5' |
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| 31550 | 3' | -53.8 | NC_006820.1 | + | 15892 | 0.67 | 0.938519 |
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Target: 5'- uGGCGCUucaAGAC-GCcGCAGGaGGCACAg -3' miRNA: 3'- -CUGCGG---UUUGaCGaCGUCUaCCGUGU- -5' |
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| 31550 | 3' | -53.8 | NC_006820.1 | + | 73552 | 0.68 | 0.928308 |
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Target: 5'- -uUGCUAGuGCUGCaacUGguGAUGGUACAg -3' miRNA: 3'- cuGCGGUU-UGACG---ACguCUACCGUGU- -5' |
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| 31550 | 3' | -53.8 | NC_006820.1 | + | 77980 | 0.69 | 0.884823 |
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Target: 5'- uAUGCUAgcAACUGCUGCAGAuauUGuaGCAg -3' miRNA: 3'- cUGCGGU--UUGACGACGUCU---ACcgUGU- -5' |
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| 31550 | 3' | -53.8 | NC_006820.1 | + | 21591 | 0.78 | 0.390617 |
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Target: 5'- cACGCCAAcuGCccccaUGCUGCAGAUgGGCACGu -3' miRNA: 3'- cUGCGGUU--UG-----ACGACGUCUA-CCGUGU- -5' |
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| 31550 | 3' | -53.8 | NC_006820.1 | + | 21832 | 1.09 | 0.004877 |
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Target: 5'- cGACGCCAAACUGCUGCAGAUGGCACAu -3' miRNA: 3'- -CUGCGGUUUGACGACGUCUACCGUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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