miRNA: 3'- gAGGGUAACGGggau----AUGACUAGAGU- -5'
miRNA: 3'- -GAGGGUaACG---------GGGAUAUGACUAG---AGU- -5'
miRNA: 3'- gaGGGUA--ACGGGGAUAUgACUaGAGU- -5'
miRNA: 3'- -GAGGGUAACGGGGAUA----UGACUa-GAGU- -5'
miRNA: 3'- -GAGGGUAACGGGGAUAUGACUAGAGU- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31551 | 5' | -51.2 | NC_006820.1 | + | 159499 | 0.66 | 0.994903 |
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Target: 5'- aUCUUAUUGCUagguauauUACUGAUCUCAu -3' miRNA: 3'- gAGGGUAACGGggau----AUGACUAGAGU- -5' |
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| 31551 | 5' | -51.2 | NC_006820.1 | + | 17043 | 0.7 | 0.944923 |
|
Target: 5'- aCUCCUAcUGCcgcccgagaCCCUAUACUGAUUacaUCAg -3' miRNA: 3'- -GAGGGUaACG---------GGGAUAUGACUAG---AGU- -5' |
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| 31551 | 5' | -51.2 | NC_006820.1 | + | 24060 | 0.71 | 0.919014 |
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Target: 5'- -cCCCAUuuUGCCCCUAUAaUGAcUUCAg -3' miRNA: 3'- gaGGGUA--ACGGGGAUAUgACUaGAGU- -5' |
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| 31551 | 5' | -51.2 | NC_006820.1 | + | 21764 | 0.85 | 0.262206 |
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Target: 5'- cCUCCCAUUGCCCCUAUaaugACUGAagUUCAa -3' miRNA: 3'- -GAGGGUAACGGGGAUA----UGACUa-GAGU- -5' |
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| 31551 | 5' | -51.2 | NC_006820.1 | + | 22033 | 1.09 | 0.009044 |
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Target: 5'- gCUCCCAUUGCCCCUAUACUGAUCUCAg -3' miRNA: 3'- -GAGGGUAACGGGGAUAUGACUAGAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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