Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31553 | 3' | -53 | NC_006820.1 | + | 23119 | 0.66 | 0.983908 |
Target: 5'- cCAUC-CUCUC-CCUgCUgCUGUgGCAg -3' miRNA: 3'- -GUAGuGAGAGuGGAgGAgGAUAgCGU- -5' |
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31553 | 3' | -53 | NC_006820.1 | + | 55205 | 0.66 | 0.981958 |
Target: 5'- aCAgCAUUUUCACCUUCUCCgAUgGUAu -3' miRNA: 3'- -GUaGUGAGAGUGGAGGAGGaUAgCGU- -5' |
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31553 | 3' | -53 | NC_006820.1 | + | 17213 | 0.67 | 0.972358 |
Target: 5'- uUAUCACUaCUCucuggaCUCCUCUgGUCGUAa -3' miRNA: 3'- -GUAGUGA-GAGug----GAGGAGGaUAGCGU- -5' |
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31553 | 3' | -53 | NC_006820.1 | + | 137544 | 0.67 | 0.972358 |
Target: 5'- uGUCAUUCUCGCUuuUCCUCCUuuUCu-- -3' miRNA: 3'- gUAGUGAGAGUGG--AGGAGGAu-AGcgu -5' |
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31553 | 3' | -53 | NC_006820.1 | + | 59771 | 0.69 | 0.933448 |
Target: 5'- --aCAUUCUCACaaCCUCCUggCGCu -3' miRNA: 3'- guaGUGAGAGUGgaGGAGGAuaGCGu -5' |
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31553 | 3' | -53 | NC_006820.1 | + | 22322 | 1.09 | 0.007207 |
Target: 5'- cCAUCACUCUCACCUCCUCCUAUCGCAu -3' miRNA: 3'- -GUAGUGAGAGUGGAGGAGGAUAGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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