Results 1 - 11 of 11 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31556 | 5' | -46.1 | NC_006820.1 | + | 24249 | 0.66 | 0.999965 |
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Target: 5'- cUGCCCCc-UAGUGG--UCGGUUCAc- -3' miRNA: 3'- -ACGGGGauAUUACUgaAGUCAAGUuu -5' |
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| 31556 | 5' | -46.1 | NC_006820.1 | + | 24283 | 0.67 | 0.999936 |
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Target: 5'- cGCCUCUA----GACUucucUCAGUUCAAc -3' miRNA: 3'- aCGGGGAUauuaCUGA----AGUCAAGUUu -5' |
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| 31556 | 5' | -46.1 | NC_006820.1 | + | 11573 | 0.67 | 0.999932 |
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Target: 5'- -uCCCCUGUGGUGGaggagugugcCAGUUCAAGg -3' miRNA: 3'- acGGGGAUAUUACUgaa-------GUCAAGUUU- -5' |
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| 31556 | 5' | -46.1 | NC_006820.1 | + | 143418 | 0.72 | 0.993158 |
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Target: 5'- uUGCCCCUGUAAUacaGAUUUCuuUUCGGGg -3' miRNA: 3'- -ACGGGGAUAUUA---CUGAAGucAAGUUU- -5' |
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| 31556 | 5' | -46.1 | NC_006820.1 | + | 23728 | 0.72 | 0.990851 |
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Target: 5'- cUGCCUgUAgGAUaACUUCAGUUCAAAc -3' miRNA: 3'- -ACGGGgAUaUUAcUGAAGUCAAGUUU- -5' |
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| 31556 | 5' | -46.1 | NC_006820.1 | + | 23440 | 0.73 | 0.977721 |
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Target: 5'- cUGaCCCUGUAGgauaACUUCAGUUCAAAc -3' miRNA: 3'- -ACgGGGAUAUUac--UGAAGUCAAGUUU- -5' |
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| 31556 | 5' | -46.1 | NC_006820.1 | + | 22955 | 0.75 | 0.962136 |
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Target: 5'- cGUgCCUAUAAUGACUgaAGUUCAAc -3' miRNA: 3'- aCGgGGAUAUUACUGAagUCAAGUUu -5' |
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| 31556 | 5' | -46.1 | NC_006820.1 | + | 21515 | 0.75 | 0.962136 |
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Target: 5'- cGUgCCUAUAAUGACUgaAGUUCAAc -3' miRNA: 3'- aCGgGGAUAUUACUGAagUCAAGUUu -5' |
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| 31556 | 5' | -46.1 | NC_006820.1 | + | 22040 | 0.87 | 0.436512 |
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Target: 5'- uUGCCCCUAUAcUGAUcUCAGUUCAAAc -3' miRNA: 3'- -ACGGGGAUAUuACUGaAGUCAAGUUU- -5' |
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| 31556 | 5' | -46.1 | NC_006820.1 | + | 21771 | 0.91 | 0.291265 |
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Target: 5'- uUGCCCCUAUAAUGACUgaAGUUCAAAc -3' miRNA: 3'- -ACGGGGAUAUUACUGAagUCAAGUUU- -5' |
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| 31556 | 5' | -46.1 | NC_006820.1 | + | 24067 | 1.07 | 0.035557 |
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Target: 5'- uUGCCCCUAUAAUGACUUCAGUUCAAAc -3' miRNA: 3'- -ACGGGGAUAUUACUGAAGUCAAGUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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