Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31560 | 3' | -43.8 | NC_006820.1 | + | 108587 | 0.68 | 0.999994 |
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Target: 5'- uAGCAgaugCUACAUCAGCAgCAacagagUCAu -3' miRNA: 3'- -UCGUa---GAUGUAGUCGU-GUaaaa--AGUu -5' |
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| 31560 | 3' | -43.8 | NC_006820.1 | + | 145114 | 0.68 | 0.999988 |
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Target: 5'- uGCGUCUGCAagAGUACAUUa----- -3' miRNA: 3'- uCGUAGAUGUagUCGUGUAAaaaguu -5' |
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| 31560 | 3' | -43.8 | NC_006820.1 | + | 86505 | 0.72 | 0.999202 |
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Target: 5'- uAGCGUCUGCAUCAucGUACuc--UUCAAa -3' miRNA: 3'- -UCGUAGAUGUAGU--CGUGuaaaAAGUU- -5' |
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| 31560 | 3' | -43.8 | NC_006820.1 | + | 18302 | 0.73 | 0.9973 |
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Target: 5'- uAGCAaUUGCAUCAGCAUAacauucuUUUUUCAu -3' miRNA: 3'- -UCGUaGAUGUAGUCGUGU-------AAAAAGUu -5' |
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| 31560 | 3' | -43.8 | NC_006820.1 | + | 70062 | 0.78 | 0.959608 |
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Target: 5'- cAGCAUCUACAUCAGUgcugGCAUUUg---- -3' miRNA: 3'- -UCGUAGAUGUAGUCG----UGUAAAaaguu -5' |
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| 31560 | 3' | -43.8 | NC_006820.1 | + | 69802 | 1.07 | 0.055726 |
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Target: 5'- cAGCAUCUACAUCAGCACAUUUUUCAAu -3' miRNA: 3'- -UCGUAGAUGUAGUCGUGUAAAAAGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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