Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31561 | 5' | -51.9 | NC_006820.1 | + | 88321 | 0.66 | 0.987866 |
Target: 5'- gAGCACC-GCA-AGGUCccuACAGCGUc- -3' miRNA: 3'- -UCGUGGaCGUcUUCAGu--UGUCGCAuu -5' |
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31561 | 5' | -51.9 | NC_006820.1 | + | 73027 | 0.66 | 0.986243 |
Target: 5'- cAGCACgaauCUGCAGAgcaccAGUCAcgACAGCa--- -3' miRNA: 3'- -UCGUG----GACGUCU-----UCAGU--UGUCGcauu -5' |
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31561 | 5' | -51.9 | NC_006820.1 | + | 93215 | 0.69 | 0.942815 |
Target: 5'- gGGCGCCc-CAGAGGUCAaugagACAGuCGUAGc -3' miRNA: 3'- -UCGUGGacGUCUUCAGU-----UGUC-GCAUU- -5' |
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31561 | 5' | -51.9 | NC_006820.1 | + | 71470 | 0.69 | 0.921877 |
Target: 5'- cAGCACUUGCAGAcGUagaAGCAGCc--- -3' miRNA: 3'- -UCGUGGACGUCUuCAg--UUGUCGcauu -5' |
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31561 | 5' | -51.9 | NC_006820.1 | + | 138133 | 0.72 | 0.835222 |
Target: 5'- uAGCAUCaGCAGGAGUCGaaACAGCugcuGUGAg -3' miRNA: 3'- -UCGUGGaCGUCUUCAGU--UGUCG----CAUU- -5' |
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31561 | 5' | -51.9 | NC_006820.1 | + | 78476 | 1.06 | 0.010533 |
Target: 5'- aAGCACCUGCAGAAGUCAACAGCGUAAc -3' miRNA: 3'- -UCGUGGACGUCUUCAGUUGUCGCAUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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