Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31562 | 3' | -44.7 | NC_006820.1 | + | 40016 | 0.66 | 1 |
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Target: 5'- uCCUAGAACG--GGCGUAaCUggCACUg -3' miRNA: 3'- cGGGUCUUGUuuUCGUAUaGAa-GUGA- -5' |
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| 31562 | 3' | -44.7 | NC_006820.1 | + | 1762 | 0.67 | 0.999994 |
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Target: 5'- ---aAGaAGCAGAAGCAaAUCUUCGCa -3' miRNA: 3'- cgggUC-UUGUUUUCGUaUAGAAGUGa -5' |
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| 31562 | 3' | -44.7 | NC_006820.1 | + | 169026 | 0.67 | 0.999994 |
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Target: 5'- -aCCAGAugGuucuAGAGCAUAUCUgUCAa- -3' miRNA: 3'- cgGGUCUugU----UUUCGUAUAGA-AGUga -5' |
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| 31562 | 3' | -44.7 | NC_006820.1 | + | 90045 | 0.68 | 0.999989 |
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Target: 5'- uGCCauuugaAGGAUggGAGCAaagcacaaaUAUCUUCAUUc -3' miRNA: 3'- -CGGg-----UCUUGuuUUCGU---------AUAGAAGUGA- -5' |
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| 31562 | 3' | -44.7 | NC_006820.1 | + | 24319 | 0.7 | 0.999723 |
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Target: 5'- aCCCAuGACcGAAGCAUAcgcgaUCUUCACc -3' miRNA: 3'- cGGGUcUUGuUUUCGUAU-----AGAAGUGa -5' |
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| 31562 | 3' | -44.7 | NC_006820.1 | + | 32383 | 0.7 | 0.999648 |
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Target: 5'- uUCCAGuAGCAAGAGUGgaAUCUUCACc -3' miRNA: 3'- cGGGUC-UUGUUUUCGUa-UAGAAGUGa -5' |
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| 31562 | 3' | -44.7 | NC_006820.1 | + | 113992 | 1.12 | 0.030788 |
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Target: 5'- cGCCCAGAACAAAAGCAUAUCUUCACUu -3' miRNA: 3'- -CGGGUCUUGUUUUCGUAUAGAAGUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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