Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31563 | 3' | -43.2 | NC_006820.1 | + | 103433 | 0.66 | 1 |
Target: 5'- aCUUCAAuuucuuggcgaggucAGGGAuCUCACCAugguUCUu -3' miRNA: 3'- gGAAGUU---------------UUCCUuGAGUGGUuu--AGAu -5' |
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31563 | 3' | -43.2 | NC_006820.1 | + | 102338 | 0.66 | 1 |
Target: 5'- gUCUUCAAAA-GAAUcCGCUAGAUCUu -3' miRNA: 3'- -GGAAGUUUUcCUUGaGUGGUUUAGAu -5' |
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31563 | 3' | -43.2 | NC_006820.1 | + | 78885 | 0.67 | 1 |
Target: 5'- aCUUCuaAGAAGGAACUUGCUgaggAGGUCa- -3' miRNA: 3'- gGAAG--UUUUCCUUGAGUGG----UUUAGau -5' |
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31563 | 3' | -43.2 | NC_006820.1 | + | 99095 | 0.66 | 1 |
Target: 5'- uUCUUac-AAGGAGCUCGCCA--UUUAu -3' miRNA: 3'- -GGAAguuUUCCUUGAGUGGUuuAGAU- -5' |
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31563 | 3' | -43.2 | NC_006820.1 | + | 104340 | 0.66 | 1 |
Target: 5'- cCCUcccCAGAAGGAGgaCGCCAAGacuUCUc -3' miRNA: 3'- -GGAa--GUUUUCCUUgaGUGGUUU---AGAu -5' |
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31563 | 3' | -43.2 | NC_006820.1 | + | 172163 | 0.66 | 1 |
Target: 5'- aCUUCA--AGGAAUUCAUCAGuuuGUCc- -3' miRNA: 3'- gGAAGUuuUCCUUGAGUGGUU---UAGau -5' |
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31563 | 3' | -43.2 | NC_006820.1 | + | 92703 | 0.68 | 0.999999 |
Target: 5'- gCCUUCGGAAGaaGAGCagaaaACUAGAUCUGg -3' miRNA: 3'- -GGAAGUUUUC--CUUGag---UGGUUUAGAU- -5' |
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31563 | 3' | -43.2 | NC_006820.1 | + | 160250 | 0.69 | 0.999996 |
Target: 5'- aUCUUCAucAGGAAgaucuauuucUUCAuCCAAAUCUAa -3' miRNA: 3'- -GGAAGUuuUCCUU----------GAGU-GGUUUAGAU- -5' |
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31563 | 3' | -43.2 | NC_006820.1 | + | 14340 | 0.76 | 0.994946 |
Target: 5'- aCUUCAAGAGaGAACUCucCCAuucuGUCUAu -3' miRNA: 3'- gGAAGUUUUC-CUUGAGu-GGUu---UAGAU- -5' |
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31563 | 3' | -43.2 | NC_006820.1 | + | 24696 | 0.8 | 0.953605 |
Target: 5'- aCCUUCAccagagaguAGAGGGGCUCACCAGGa--- -3' miRNA: 3'- -GGAAGU---------UUUCCUUGAGUGGUUUagau -5' |
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31563 | 3' | -43.2 | NC_006820.1 | + | 145869 | 1.11 | 0.050775 |
Target: 5'- uCCUUCAAAAGGAACUCACCAAAUCUAg -3' miRNA: 3'- -GGAAGUUUUCCUUGAGUGGUUUAGAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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