Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31564 | 5' | -50.1 | NC_006820.1 | + | 45002 | 0.66 | 0.996547 |
Target: 5'- --aUAGCAUCAcuaauagauUCUGGUCUUGaUGGa -3' miRNA: 3'- guaGUCGUAGU---------AGACCAGGACaACUa -5' |
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31564 | 5' | -50.1 | NC_006820.1 | + | 123493 | 0.67 | 0.995245 |
Target: 5'- uGUCGGCAcUCcugCUGGUCaaGUUGGUg -3' miRNA: 3'- gUAGUCGU-AGua-GACCAGgaCAACUA- -5' |
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31564 | 5' | -50.1 | NC_006820.1 | + | 156949 | 0.67 | 0.995245 |
Target: 5'- --aUAGUAUUcgaaguguaaAUgUGGUCCUGUUGAUg -3' miRNA: 3'- guaGUCGUAG----------UAgACCAGGACAACUA- -5' |
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31564 | 5' | -50.1 | NC_006820.1 | + | 156754 | 1.07 | 0.015429 |
Target: 5'- cCAUCAGCAUCAUCUGGUCCUGUUGAUg -3' miRNA: 3'- -GUAGUCGUAGUAGACCAGGACAACUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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