Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31565 | 5' | -51.2 | NC_006820.1 | + | 60002 | 0.67 | 0.985437 |
Target: 5'- -cAUCUGGUGuuauuucuAGCACgaGACUCGc -3' miRNA: 3'- gaUAGACCACuu------UCGUGggCUGAGUa -5' |
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31565 | 5' | -51.2 | NC_006820.1 | + | 24868 | 0.7 | 0.945755 |
Target: 5'- -gGUCUGGUGAGAGguCUCGGuCUUg- -3' miRNA: 3'- gaUAGACCACUUUCguGGGCU-GAGua -5' |
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31565 | 5' | -51.2 | NC_006820.1 | + | 61095 | 0.71 | 0.925631 |
Target: 5'- uCUAUCUGGUGAuaAAGUACCagcACUUAa -3' miRNA: 3'- -GAUAGACCACU--UUCGUGGgc-UGAGUa -5' |
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31565 | 5' | -51.2 | NC_006820.1 | + | 163306 | 1.07 | 0.01289 |
Target: 5'- gCUAUCUGGUGAAAGCACCCGACUCAUa -3' miRNA: 3'- -GAUAGACCACUUUCGUGGGCUGAGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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