Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
31571 | 5' | -50 | NC_006852.1 | + | 170 | 0.67 | 0.766952 |
Target: 5'- gUGACG-UGuccaUUCGGCGCCAGCa-- -3' miRNA: 3'- aACUGCuACug--AAGUCGUGGUUGaga -5' |
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31571 | 5' | -50 | NC_006852.1 | + | 6512 | 0.67 | 0.732752 |
Target: 5'- -gGugGAuauagcUGACgcaUCAGCACCA-CUCg -3' miRNA: 3'- aaCugCU------ACUGa--AGUCGUGGUuGAGa -5' |
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31571 | 5' | -50 | NC_006852.1 | + | 12916 | 0.7 | 0.576945 |
Target: 5'- aUUGACGAccUGAgUUCucaacuucugaGGCAUCAACUCa -3' miRNA: 3'- -AACUGCU--ACUgAAG-----------UCGUGGUUGAGa -5' |
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31571 | 5' | -50 | NC_006852.1 | + | 4273 | 1.07 | 0.002082 |
Target: 5'- gUUGACGAUGACUUCAGCACCAACUCUg -3' miRNA: 3'- -AACUGCUACUGAAGUCGUGGUUGAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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