Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
31572 | 5' | -51.9 | NC_006852.1 | + | 10570 | 0.67 | 0.653515 |
Target: 5'- gGCAGAC-CCCaggGAGCCUU--CCAUGUu -3' miRNA: 3'- -CGUUUGcGGGa--CUCGGAAguGGUAUA- -5' |
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31572 | 5' | -51.9 | NC_006852.1 | + | 16482 | 0.68 | 0.58211 |
Target: 5'- ---uAUGUCCUGAGUgugUCACCAUAUg -3' miRNA: 3'- cguuUGCGGGACUCGga-AGUGGUAUA- -5' |
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31572 | 5' | -51.9 | NC_006852.1 | + | 30437 | 0.75 | 0.237807 |
Target: 5'- uGCAGACcuUCCUGAGCCUUCAaCAUAa -3' miRNA: 3'- -CGUUUGc-GGGACUCGGAAGUgGUAUa -5' |
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31572 | 5' | -51.9 | NC_006852.1 | + | 4388 | 1.07 | 0.001132 |
Target: 5'- gGCAAACGCCCUGAGCCUUCACCAUAUc -3' miRNA: 3'- -CGUUUGCGGGACUCGGAAGUGGUAUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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