miRNA: 3'- cCAUGGUG--UACaAUCAUA-AUGG-GACG- -5'
miRNA: 3'- cCAUGGuguACA--AUCAUAAUGGgACg -5'
miRNA: 3'- -CCAUGGUGUACA----AUC----AUAAugGg--ACG- -5'
miRNA: 3'- cCAUGGUGUACaAUCa-----UAAUGG-GACg -5'
miRNA: 3'- -CCAUGGUGUACAAUCAUAAUGGGACG- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31592 | 3' | -45.1 | NC_006852.1 | + | 17255 | 0.66 | 0.99053 |
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Target: 5'- uGUACUGCucGUG-UGGUGUaUACCgCUGCu -3' miRNA: 3'- cCAUGGUG--UACaAUCAUA-AUGG-GACG- -5' |
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| 31592 | 3' | -45.1 | NC_006852.1 | + | 14254 | 0.66 | 0.988963 |
|
Target: 5'- aGUGCCu--UGUugUGGUGUUGCCgUGg -3' miRNA: 3'- cCAUGGuguACA--AUCAUAAUGGgACg -5' |
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| 31592 | 3' | -45.1 | NC_006852.1 | + | 5558 | 0.66 | 0.987202 |
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Target: 5'- uGGUGCCACAUGUgauuUGGaauuUAUUugUaaaUGUg -3' miRNA: 3'- -CCAUGGUGUACA----AUC----AUAAugGg--ACG- -5' |
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| 31592 | 3' | -45.1 | NC_006852.1 | + | 20405 | 0.68 | 0.960303 |
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Target: 5'- aGUGCCACGUGcUAGagcuucAUUACCgCUGa -3' miRNA: 3'- cCAUGGUGUACaAUCa-----UAAUGG-GACg -5' |
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| 31592 | 3' | -45.1 | NC_006852.1 | + | 21888 | 1.17 | 0.002001 |
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Target: 5'- aGGUACCACAUGUUAGUAUUACCCUGCu -3' miRNA: 3'- -CCAUGGUGUACAAUCAUAAUGGGACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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