Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31600 | 3' | -55.7 | NC_006875.1 | + | 4821 | 0.66 | 0.186013 |
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Target: 5'- aUGGG-CugGGCCUCGUGaucccacacggagcGCCGgggGCCa -3' miRNA: 3'- cACCCuGugCUGGGGCGU--------------UGGUa--UGG- -5' |
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| 31600 | 3' | -55.7 | NC_006875.1 | + | 275 | 0.67 | 0.155489 |
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Target: 5'- cUGGGAgGCuuGACCCUGuCAGCCAc--- -3' miRNA: 3'- cACCCUgUG--CUGGGGC-GUUGGUaugg -5' |
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| 31600 | 3' | -55.7 | NC_006875.1 | + | 6371 | 0.67 | 0.14131 |
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Target: 5'- aUGGG-CACguuggGACCCUGCGGgcCCAcGCCa -3' miRNA: 3'- cACCCuGUG-----CUGGGGCGUU--GGUaUGG- -5' |
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| 31600 | 3' | -55.7 | NC_006875.1 | + | 2670 | 0.68 | 0.120289 |
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Target: 5'- -cGGGcaGgACGACCCCGU---CGUGCCa -3' miRNA: 3'- caCCC--UgUGCUGGGGCGuugGUAUGG- -5' |
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| 31600 | 3' | -55.7 | NC_006875.1 | + | 4388 | 0.68 | 0.120289 |
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Target: 5'- uGUGGGcacCGCGACgCCCuCGACCAagGCUg -3' miRNA: 3'- -CACCCu--GUGCUG-GGGcGUUGGUa-UGG- -5' |
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| 31600 | 3' | -55.7 | NC_006875.1 | + | 6305 | 0.75 | 0.031636 |
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Target: 5'- uGUGGGACAUaGCCCUGCAuaACCuggacgGCCc -3' miRNA: 3'- -CACCCUGUGcUGGGGCGU--UGGua----UGG- -5' |
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| 31600 | 3' | -55.7 | NC_006875.1 | + | 2129 | 1.13 | 2.5e-05 |
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Target: 5'- cGUGGGACACGACCCCGCAACCAUACCa -3' miRNA: 3'- -CACCCUGUGCUGGGGCGUUGGUAUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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