Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31604 | 5' | -55.2 | NC_006875.1 | + | 6074 | 0.66 | 0.185489 |
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Target: 5'- uGCGGCAGaACCuggccggcgaugACGGCGccGCCGUCc-- -3' miRNA: 3'- -CGCCGUUgUGG------------UGCCGU--CGGUAGuug -5' |
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| 31604 | 5' | -55.2 | NC_006875.1 | + | 5096 | 0.66 | 0.179693 |
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Target: 5'- cGCGuGCGauggacgcugGCGCCAcCGGCGccGCCG-CAGCu -3' miRNA: 3'- -CGC-CGU----------UGUGGU-GCCGU--CGGUaGUUG- -5' |
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| 31604 | 5' | -55.2 | NC_006875.1 | + | 1238 | 0.66 | 0.174059 |
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Target: 5'- cGCGGCgAGCGCCAagugcUGGCuAGCaugcUCAAUg -3' miRNA: 3'- -CGCCG-UUGUGGU-----GCCG-UCGgu--AGUUG- -5' |
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| 31604 | 5' | -55.2 | NC_006875.1 | + | 1842 | 0.66 | 0.16326 |
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Target: 5'- gGUGGUGGCACCccaGGguGCUggcgcaggaaGUCGGCa -3' miRNA: 3'- -CGCCGUUGUGGug-CCguCGG----------UAGUUG- -5' |
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| 31604 | 5' | -55.2 | NC_006875.1 | + | 5140 | 0.68 | 0.125873 |
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Target: 5'- uGCGGCGGCGCCggugGCGcCAGCgucCAUCGcACg -3' miRNA: 3'- -CGCCGUUGUGG----UGCcGUCG---GUAGU-UG- -5' |
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| 31604 | 5' | -55.2 | NC_006875.1 | + | 1809 | 0.69 | 0.110303 |
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Target: 5'- uGCGcCAGCAcCCugGGguGCCAccaccugCAACg -3' miRNA: 3'- -CGCcGUUGU-GGugCCguCGGUa------GUUG- -5' |
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| 31604 | 5' | -55.2 | NC_006875.1 | + | 5525 | 0.7 | 0.084433 |
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Target: 5'- aGCGG-GAUACCGCcGCGGgCAUCAAUa -3' miRNA: 3'- -CGCCgUUGUGGUGcCGUCgGUAGUUG- -5' |
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| 31604 | 5' | -55.2 | NC_006875.1 | + | 1259 | 0.7 | 0.081363 |
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Target: 5'- uUGGCGcuCGCCGCguguggagaccguGGCAGCCAUCGu- -3' miRNA: 3'- cGCCGUu-GUGGUG-------------CCGUCGGUAGUug -5' |
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| 31604 | 5' | -55.2 | NC_006875.1 | + | 6111 | 1.13 | 2.3e-05 |
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Target: 5'- cGCGGCAACACCACGGCAGCCAUCAACa -3' miRNA: 3'- -CGCCGUUGUGGUGCCGUCGGUAGUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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