Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31606 | 3' | -48.9 | NC_006879.1 | + | 23220 | 0.68 | 0.826667 |
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Target: 5'- aCCCCCAAcAGuUGCAAGaGAGGc---GCAa -3' miRNA: 3'- -GGGGGUU-UC-ACGUUC-CUCUuaauCGU- -5' |
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| 31606 | 3' | -48.9 | NC_006879.1 | + | 19494 | 0.69 | 0.752099 |
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Target: 5'- gCCCCUGAAGgcugccaguUGCGAGaGGGAAUggagaugGGCAc -3' miRNA: 3'- -GGGGGUUUC---------ACGUUC-CUCUUAa------UCGU- -5' |
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| 31606 | 3' | -48.9 | NC_006879.1 | + | 17311 | 0.69 | 0.740749 |
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Target: 5'- aCCCCAGAG-GCcagaGAGGAGccaacUGGCAa -3' miRNA: 3'- gGGGGUUUCaCG----UUCCUCuua--AUCGU- -5' |
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| 31606 | 3' | -48.9 | NC_006879.1 | + | 25834 | 0.72 | 0.598536 |
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Target: 5'- uCCCCCGGAGcgcggcUGCGAGGaAGAGcucuGCAg -3' miRNA: 3'- -GGGGGUUUC------ACGUUCC-UCUUaau-CGU- -5' |
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| 31606 | 3' | -48.9 | NC_006879.1 | + | 9018 | 0.77 | 0.332172 |
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Target: 5'- gCCCCC---GUGCAGGGAGggUaAGUg -3' miRNA: 3'- -GGGGGuuuCACGUUCCUCuuAaUCGu -5' |
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| 31606 | 3' | -48.9 | NC_006879.1 | + | 1143 | 1.12 | 0.001475 |
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Target: 5'- gCCCCCAAAGUGCAAGGAGAAUUAGCAg -3' miRNA: 3'- -GGGGGUUUCACGUUCCUCUUAAUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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