miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
31606 3' -48.9 NC_006879.1 + 23220 0.68 0.826667
Target:  5'- aCCCCCAAcAGuUGCAAGaGAGGc---GCAa -3'
miRNA:   3'- -GGGGGUU-UC-ACGUUC-CUCUuaauCGU- -5'
31606 3' -48.9 NC_006879.1 + 19494 0.69 0.752099
Target:  5'- gCCCCUGAAGgcugccaguUGCGAGaGGGAAUggagaugGGCAc -3'
miRNA:   3'- -GGGGGUUUC---------ACGUUC-CUCUUAa------UCGU- -5'
31606 3' -48.9 NC_006879.1 + 17311 0.69 0.740749
Target:  5'- aCCCCAGAG-GCcagaGAGGAGccaacUGGCAa -3'
miRNA:   3'- gGGGGUUUCaCG----UUCCUCuua--AUCGU- -5'
31606 3' -48.9 NC_006879.1 + 25834 0.72 0.598536
Target:  5'- uCCCCCGGAGcgcggcUGCGAGGaAGAGcucuGCAg -3'
miRNA:   3'- -GGGGGUUUC------ACGUUCC-UCUUaau-CGU- -5'
31606 3' -48.9 NC_006879.1 + 9018 0.77 0.332172
Target:  5'- gCCCCC---GUGCAGGGAGggUaAGUg -3'
miRNA:   3'- -GGGGGuuuCACGUUCCUCuuAaUCGu -5'
31606 3' -48.9 NC_006879.1 + 1143 1.12 0.001475
Target:  5'- gCCCCCAAAGUGCAAGGAGAAUUAGCAg -3'
miRNA:   3'- -GGGGGUUUCACGUUCCUCUUAAUCGU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.